Diamond Octyl-HIC Resins丨Bestchrom

Diamond Octyl

Product Characteristics

HIC resin using Octyl as its ligands
High rigid agarose matrix, enables high flow rate and low back pressure
Ligands with intermediate hydrophobicity, suitable for the capture and intermediate purification of macro-biomolecules with intermediate hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.

Octyl Bestarose 4FF

Diamond Octyl Mustang

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Diamond Octyl is obtained by coupling moderately hydrophobic aliphatic octyl groups to high-rigidity agarose matrix. It enjoys low backpressure and fast flow velocity, which is suitable for large-scale purification of biomolecules on large scale.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked rigid agarose
Functional group	Octyl
Ligand concentration	~5μmol Ligand/mL resin
Particle size⁺	40~120μm
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH⁺⁺,1M HAc⁺⁺,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13 (working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min)
Storage⁺⁺⁺	2~30℃,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Diamond Octyl	AH302305	25mL
	AH302307	100mL
	AH302311	500mL
	AH302312	1L
	AH302313	5L
	AH302314	10L
	AH302315	20L
	AH302316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Diamond Octyl	EH01221	1×1mL
	EH302351	5×1mL
	EH302303	1×5mL
	EH302353	5×5mL
EzScreen Diamond Octyl	EH01225	1×4.6mL
EzScreen Diamond Octyl	EH01235	5×4.6mL
EzLoad 16/10 Diamond Octyl	EH302204	1 pcs
EzLoad 26/10 Diamond Octyl	EH302206	1 pcs