Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Octyl Mustang is obtained by coupling moderately hydrophobic aliphatic Octyl groups on fine-grained high-rigidity agarose matrix. It enjoys high resolution and low backpressure, which is suitable for the intermediate and polishing stages of protein downstream purification.
Appearance |
White slurry |
Matrix |
Highly cross-linked rigid agarose |
Functional group |
Octyl |
Average particle size |
36~44μm |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥450cm/h(0.5MPa BXK 100/500, H=20cm, 20℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH+,1M HAc+,6M GuHCl,30% isopropyl alcohol,70% ethanol |
pH stability |
2~14(CIP),3~13(working) |
Operating temperature |
Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min) |
Storage++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
90~180cm/h |
+1M NaOH and 1M HAc only be used for cleaning.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond Octyl Mustang |
AH302205 |
25mL |
AH302207 |
100mL |
|
AH302211 |
500mL |
|
AH302212 |
1L |
|
AH302213 |
5L |
|
AH302214 |
10L |
|
AH302215 |
20L |
|
AH302216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Octyl Mustang |
EH01521 |
1×1mL |
EH302251 |
5×1mL |
|
EH302203 |
1×5mL |
|
EH302253 |
5×5mL |
|
EzScreen Octyl Mustang |
EH01525 |
1×4.6mL |
EH01535 |
5×4.6mL |
|
EzLoad 16/10 Octyl Mustang |
EH01501 |
1 pcs |
EzLoad 26/10 Octyl Mustang |
EH01511 |
1 pcs |