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Phenyl HIC Resins

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Hydrophobic Interaction Resins
Phenyl HIC Resins

Diamond Phenyl(HS)

Diamond Phenyl(HS)

Product Characteristics

  • HIC resin using Phenyl as its ligands
  • High rigid agarose matrix, low back pressure and high flow rate
  • Different binding capacity and selectivity compared with Phenyl Bestarose FF resins
  • Ligands with strong hydrophobicity, suitable for the capture and intermediate purification of macro-biomolecules with weak hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.
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Description
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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Diamond Phenyl (HS) is a phenyl group with high substitution and strong hydrophobicity based on high rigid agarose. It has low back pressure and fast flow velocity. It is suitable for large-scale separation and purification of biomolecules.

2. Technical characteristics

Appearance

White slurry

Matrix

High rigidity agarose

Functional group

Phenyl

Particle size+

40~120μm

Ligand concentration

~27μmol Ligand/mL resin

Dynamic binding capacity

~27 mg BSA/mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20℃)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol

pH stability

2~14(CIP),3~13(working)

Operating temperature

Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min)

Storage+++

2~30℃, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

150~350cm/h

 

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

Diamond Phenyl(HS)

AH321305

25mL

AH321307

100mL

AH321311

500mL

AH321312

1L

AH321313

5L

AH321314

10L

AH321315

20L

AH321316

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast Diamond Phenyl (HS)

EH01021

1×1mL

EH321351

5×1mL

EH321303

1×5mL

EH321353

5×5mL

EzScreen Diamond Phenyl(HS)

EH01025

1×4.6mL

EH01035

5×4.6mL

EzLoad 16/10 Diamond Phenyl(HS)

EH321304

1 pcs

EzLoad 26/10 Diamond Phenyl(HS)

EH321306

1 pcs

 

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