Diamond Phenyl(LS)

Product Characteristics

HIC resin using low concentration of Phenyl as its ligands
High rigid agarose matrix, low back pressure and high flow rate
Different binding capacity and selectivity compared with Phenyl Bestarose FF resins
Ligands with strong hydrophobicity, suitable for the capture and intermediate purification of macro-biomolecules with weak hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.

Phenyl Bestarose HP

Diamond Phenyl Mustang

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Diamond Phenyl (LS) is based on high-rigidity agarose with low substitution and strong hydrophobic phenyl groups. It has low back pressure and fast flow velocity, which is suitable for separation and purification of biomolecules on large scale.

2. Technical characteristics

Appearance	White slurry
Matrix	High rigidity agarose
Functional group	Phenyl
Particle size⁺	40~120μm
Ligand concentration	~ 22μmol Ligand/mL resin
Dynamic binding capacity	~22 mg BSA/mL packed resin
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH⁺⁺,1M HAc⁺⁺,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min)
Storage⁺⁺⁺	2~30℃,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Diamond Phenyl(LS)	AH311305	25mL
	AH311307	100mL
	AH311311	500mL
	AH311312	1L
	AH311313	5L
	AH311314	10L
	AH311315	20L
	AH311316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Diamond Phenyl(LS)	EH01321	1×1mL
	EH311351	5×1mL
	EH311303	1×5mL
	EH311353	5×5mL
EzScreen Diamond Phenyl(LS)	EH01325	1×4.6mL
EzScreen Diamond Phenyl(LS)	EH01335	5×4.6mL
EzLoad 16/10 Diamond Phenyl(LS)	EH311304	1 pcs
EzLoad 26/10 Diamond Phenyl(LS)	EH311306	1 pcs