Diamond Phenyl Mustang-HIC Resins丨Bestchrom

Diamond Phenyl Mustang

Product Characteristics

HIC resin using Phenyl as its ligands
High rigid agarose matrix, offers better resolution while still ensuring high throughput
Ligands with strong hydrophobicity, suitable for the intermediate purification and polishing of macro-biomolecules with weak hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.

Phenyl Bestarose FF(LS)

Phenyl Bestarose FF(HS)

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Diamond Phenyl Mustang is based on fine particle high-rigidity agarose with a hydrophobic phenyl group. The high resolution and low back pressure are suitable for the intermediate purification stage and fine purification stage of protein downstream purification.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose
Functional group	Phenyl
Average particle size	36~44μm
Hydrophobicity	The retention time of Lyzosyme is 45-50min
Dynamic binding capacity	~19mg BSA/mL packed resin
Max. pressure	0.5MPa
Pressure flow velocity	≥450cm/h(0.5MPa BXK 100/500, H=20cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 2M NaOH+,0.5M NaOH+,1M HAc+, 6M GuHCl,8M Urea,30% isopropyl alcohol,30% acetonitrile,2%SDS,3M(NH₄)₂SO₄⁺ 0.1M KH₂PO₄(pH7.0)
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	90~180cm/h

+1M NaOH and 1M HAc only be used for cleaning.

++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Diamond Phenyl Mustang	AH301205	25mL
	AH301207	100mL
	AH301211	500mL
	AH301212	1L
	AH301213	5L
	AH301214	10L
	AH301215	20L
	AH301216	40L

Prepacked columns	Cat. No.	Pack size
EzFast Phenyl Mustang	EH01421	1×1mL
	EH321251	5×1mL
	EH321203	1×5mL
	EH321253	5×5mL
EzScreen Phenyl Mustang	EH01425	1×4.6mL
EzScreen Phenyl Mustang	EH01435	5×4.6mL
EzLoad 16/10 Phenyl Mustang	EH301204	1 pcs
EzLoad 26/10 Phenyl Mustang	EH301206	1 pcs