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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, through the modification and modification of traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond substrate. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond Q is a strong anion exchange resin formed by coupling quaternary ammonium groups on highly rigid agarose microspheres, which has stronger pressure resistance and higher load than Q Bestarose FF.
|
Appearance |
White slurry |
|
Matrix |
High-rigidity agarose with a long chain of dextran |
|
Functional group |
Quaternary amine group |
|
Average particle size+ |
~90μm |
|
Ionic capacity |
160~220μmol Cl-/mL packed resin |
|
Dynamic binding capacity |
> 100mg BSA/mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥1500cm/h(0.5MPa, BXK 100/500, H =20cm, 20℃) |
|
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++,70% ethanol , 20% ethanol, 6M GuHCl,8M Urea Avoid contact with oxidizing agents, cationic detergents. |
|
pH stability |
2~14(CIP), 2~12(Working) |
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Operating temperature |
Operating temperature:2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min) |
|
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommend flow velocity |
90~500cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Diamond Q |
AI0111 |
25mL |
|
AI0112 |
100mL |
|
|
AI314311 |
500mL |
|
|
AI0113 |
1L |
|
|
AI0114 |
5L |
|
|
AI0115 |
10L |
|
|
AI314315 |
20L |
|
|
AI314316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond Q |
EI01121 |
1×1mL |
|
EI314351 |
5×1mL |
|
|
EI314303 |
1×5mL |
|
|
EI314353 |
5×5mL |
|
|
EzScreen Diamond Q |
EI01125 |
1×4.6mL |
|
EI01135 |
5×4.6mL |
|
|
EzSelect Diamond Q |
EI01126 |
8×600μL |
|
EzLoad 16/10 Diamond Q |
EI314304 |
1 pcs |
|
EzLoad 26/10 Diamond Q |
EI314306 |
1 pcs |
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