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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, through the modification to traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond Q Mustang is a strong anion exchange resin formed by coupling quaternary ammonium groups to fine-grained high-rigidity agarose microspheres. It has a higher resolution than Diamond Q and is suitable for polishing or intermediate purification in the chromatography process.
|
Appearance |
White slurry |
|
Matrix |
High rigidity agarose |
|
Functional group |
Quaternary amine group |
|
Average particle size+ |
36~44μm |
|
Ionic capacity |
150~180μmol Cl-/mL packed resin |
|
Dynamic binding capacity |
> 50 mg β -lactoglobulin /mL packed resin > 55mg BSA/mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥450cm/h(0.5MPa ,BXK 100/500, H=20cm,20℃) |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++,70% ethanol , 20% ethanol, 30% isopropyl alcohol, 6M GuHCl,8M Urea, 100mM thionol +25mM Tris pH8.5, 0.1M citric acid +8M urea +1M NaCl pH2.5, 0.1M citric acid +8M urea pH2.5. Avoid contact with oxidizing agents, cationic detergents. |
|
pH stability |
2~14 (CIP), 2~12(Working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
|
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol. |
|
Recommended flow velocity |
100~300cm/h |
+The average particle size is the cumulative resin particle size of the resin volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Diamond Q Mustang |
AI0171 |
25mL |
|
AI0172 |
100mL |
|
|
AI304211 |
500mL |
|
|
AI0173 |
1L |
|
|
AI0174 |
5L |
|
|
AI0175 |
10L |
|
|
AI304215 |
20L |
|
|
AI304216 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Q Mustang |
EI01721 |
1×1mL |
|
EI304251 |
5×1mL |
|
|
EI304203 |
1×5mL |
|
|
EI304253 |
5×5mL |
|
|
EzScreen Q Mustang |
EI01725 |
1×4.6mL |
|
EI01735 |
5×4.6mL |
|
|
EzLoad 16/10 Q Mustang |
EI304204 |
1 pcs |
|
EzLoad 26/10 Q Mustang |
EI304206 |
1 pcs |
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