Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, through the modification and modification of traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond substrate. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond Q Mustang XL is a high-rigidity agarose microsphere first coupled with a long dextran chain, and then cross-linked quaternary amino groups on the dextran molecule. This structure reduces the steric hindrance effect between biomolecules and ligands, greatly increasing the DBC of target molecules.
Appearance |
White slurry |
Matrix |
High-rigidity agarose with long dextran chain |
Functional group |
Quaternary amine group |
Average particle size+ |
36~44μm |
Ionic capacity |
170-230 μmol Cl-/mL packed resin |
Dynamic binding capacity |
> 100mg BSA/ mL packed resin |
Max. pressure |
0.5MPa |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
pH stability |
2~14(CIP), 2~12 (working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage+++ |
2~30℃,20% ethanol or 2% benzyl alcohol |
Recommend flow velocity |
100~300cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond Q Mustang XL |
AI314205 |
25mL |
AI314207 |
100mL |
|
AI314211 |
500mL |
|
AI314212 |
1L |
|
AI314213 |
5L |
|
AI314214 |
10L |
|
AI314215 |
20L |
|
AI314216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Q Mustang XL |
EI03721 |
1×1mL |
EI314251 |
5×1mL |
|
EI314203 |
1×5mL |
|
EI314253 |
5×5mL |
|
EzScreen Q Mustang XL |
EI03725 |
1×4.6mL |
EI03735 |
5×4.6mL |
|
EzLoad 16/10 Q Mustang XL |
EI314204 |
1 pcs |
EzLoad 26/10 Q Mustang XL |
EI314206 |
1 pcs |