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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond SP is a strong cation exchange resin formed by coupling sulfopropyl on high rigidity agarose microspheres, which has better pressure resistance and higher binding capacity than SP Bestarose FF.
|
Appearance |
White slurry |
|
Matrix |
High rigidity agarose attached by long chains of dextran |
|
Functional group |
Sulfopropyl |
|
Average particle size+ |
~90μm |
|
Ionic capacity |
170~220μmol H+/mL packed resin |
|
Dynamic binding capacity |
> 100 mg lysozyme/mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥1500cm/h(0.5MPa, BXK 100/500, H=20cm, 20℃) |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++, 1M HAc++ ,6M GuHCl, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
|
pH stability |
3~14(CIP),4~13(working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
|
Storage+++ |
2~30℃, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc |
|
Recommended flow velocity |
90~500cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Diamond SP |
AI319305 |
25mL |
|
AI319307 |
100mL |
|
|
AI319311 |
500mL |
|
|
AI319312 |
1L |
|
|
AI319313 |
5L |
|
|
AI319314 |
10L |
|
|
AI319315 |
20L |
|
|
AI319316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond SP |
EI04521 |
1×1mL |
|
EI04531 |
5×1mL |
|
|
EI04523 |
1×5mL |
|
|
EI04533 |
5×5mL |
|
|
EzScreen Diamond SP |
EI04525 |
1×4.6mL |
|
EI04535 |
5×4.6mL |
|
|
EzLoad 16/10 Diamond SP |
EI04501 |
1 pcs |
|
EzLoad 26/10 Diamond SP |
EI04511 |
1 pcs |
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