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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, through the modification to traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond SP Mustang is a strong cation exchange resin formed by coupling sulfopropyl to a fine, high rigidity agarose microsphere with a higher resolution than Diamond SP, which is suitable for fine or medium purification in the chromatography process.
|
Appearance |
White slurry |
|
Matrix |
High rigidity agarose |
|
Functional group |
Sulfopropyl |
|
Average particle size+ |
36-44μm |
|
Ionic capacity |
130~160μmol H+/mL packed resin |
|
Dynamic binding capacity |
> 70 mg lysozyme/mL packed resin > 95mg BSA/mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥450cm/h(0.5MPa, BXK100/500, H=20cm,20℃) |
|
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAC++,70% ethanol, 20% ethanol, 6M GuHCl, 8M Urea, 100mM thioglycerol+25mM Tris pH8.5, 0.1M citric acid+8M urea+1M NaCl pH2.5 Avoid contact with oxidizing agents, cationic detergents. |
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pH stability |
3~14(CIP), 4~12 (Working) |
|
Operating temperature |
Operating temperature:2~40℃, Can't freeze, Can tolerate 121℃ autoclaving (20 min) |
|
Storage+++ |
2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc |
|
Recommend flow velocity |
100~300cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Diamond SP Mustang |
AI0191 |
25mL |
|
AI0192 |
100mL |
|
|
AI303211 |
500mL |
|
|
AI0193 |
1L |
|
|
AI0194 |
5L |
|
|
AI0195 |
10L |
|
|
AI303215 |
20L |
|
|
AI303216 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast SP Mustang |
EI01921 |
1×1mL |
|
EI303251 |
5×1mL |
|
|
EI303203 |
1×5mL |
|
|
EI303253 |
5×5mL |
|
|
EzScreen SP Mustang |
EI01925 |
1×4.6mL |
|
EI01935 |
5×4.6mL |
|
|
EzLoad 16/10 SP Mustang |
EI303204 |
1 pcs |
|
EzLoad 26/10 SP Mustang |
EI303206 |
1 pcs |
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