Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, through the modification to traditional agarose molecules, it has stronger mechanical properties, which is called high rigid agarose, namely Diamond matrix. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Based on a high-rigidity agarose microsphere, Diamond SP Mustang XL is obtained by firstly coupling dextran long chain, and then cross-linking sulfopropyl groups on the dextran molecules. This structure reduces the steric hindrance effect between biomolecules and ligands, greatly increasing the dynamic binding capacity of the target molecules.
Appearance |
White slurry, can be layered |
Matrix |
High-rigidity agarose with long dextran chain |
Functional group |
Sulfopropyl |
Average particle size+ |
36~44μm |
Ionic capacity |
160~220μmol H+/mL packed resin |
Dynamic binding capacity |
> 100 mg lysozyme/mL packed resin |
Max. pressure |
0.5MPa |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
pH stability |
3~14(CIP), 4~12(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage+++ |
2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc |
Recommend flow velocity |
100~300cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond SP Mustang XL |
AI313205 |
25mL |
AI313207 |
100mL |
|
AI313211 |
500mL |
|
AI313212 |
1L |
|
AI313213 |
5L |
|
AI313214 |
10L |
|
AI313215 |
20L |
|
AI313216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast SP Mustang XL |
EI03821 |
1×1mL |
EI313251 |
5×1mL |
|
EI313203 |
1×5mL |
|
EI313253 |
5×5mL |
|
EzScreen SP Mustang XL |
EI03825 |
1×4.6mL |
EI03835 |
5×4.6mL |
|
EzLoad 16/10 SP Mustang XL |
EI313204 |
1 pcs |
EzLoad 26/10 SP Mustang XL |
EI313206 |
1 pcs |