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IgM Capture Bestarose HP is a thiophilic affinity resin made by fixing the sulfur-containing compound 2-mercapyridine on fine particles of high rigidity agarose. Its optimized ligand density has an appropriate affinity with IgM while its fine particles can increase the binding capacity of IgM with larger molecular weight. Thiophilic affinity works by the interaction between electron donor and electron accepter to isolate and purify biomolecules, which is strengthened in high-salt environments and weakened in low-salt environments.
|
Appearance |
White slurry |
|
Matrix |
Highly cross-linked agarose, 6% |
|
Particle size |
24~44μm (the average particle size is 34μm) |
|
Functional group |
2-mercaptopyridine |
|
Ligand concentration |
~2mg 2- mercaptopyridine /mL resin |
|
Dynamic binding capacity |
5mg human IgM/mL resin |
|
Chemical Stability |
Stable in common aqueous buffers:30% isopropyl alcohol,70% ethanol,1M HAC+,0.1M NaOH |
|
Max. pressure |
0.3MPa |
|
pH stability |
2~13(CIP),3~11(Working) |
|
Storage++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
+ 1M HAc only be used for cleaning.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
IgM Capture Bestarose HP |
AA0152 |
25mL |
|
AA0153 |
100mL |
|
|
AA0154 |
500mL |
|
|
AA0155 |
1L |
|
|
AA0156 |
5L |
|
|
AA0157 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast IgM HP |
EA01521 |
1×1mL |
|
EA01531 |
5×1mL |
|
|
EA01523 |
1×5mL |
|
|
EA01533 |
5×5mL |
|
|
EzScreen IgM HP |
EA01525 |
1×4.6mL |
|
EA01535 |
5×4.6mL |
|
|
EzLoad 16/10 IgM HP |
EA01501 |
1 pcs |
|
EzLoad 26/10 IgM HP |
EA01511 |
1 pcs |
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