Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecules. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and number difference of biological molecules under specific conditions to separate them. It has the characteristics of high binding capacity, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
MegaPoly BXS is a strong cation IEX resin, obtained by coupling the sulfopropyl functional groups on the cross-linked Polystyrene-divinylbenzene base matrix. With an average particle size of 50 μm, the resin possesses a special pore structure, which offers larger pores for flow-through and diffusion. Thus, it can not only provide high flow velocity without compromising bio-molecule binding capacity and selectivity, but also ensure rapid interaction between targeted bio-molecules and functional groups, granting it merits such as high flow velocity, high binding capacity and high resolution. Accordingly, the resin is widely applied in the efficient purification of micro-bio molecules including recombinant protein, monoclonal antibodies, DNA, viruses and peptides, providing it with a promising prospect in the bio-pharmaceutical industry.
The resin has the following features:
● The cross-linked base matrix has strong mechanical resistance and low back-pressure
● Special pore structure enhances interaction between bio-molecules and resin
● High binding capacity, high resolution and fast flow
● Physical and chemical stability
● Option for more efficient purification and separation
Appearance |
White slurry |
Matrix |
Cross-linked polystyrene-divinylbenzene beads |
Functional Group |
Sulfopropyl |
Particle size |
~50μm |
Dynamic binding capacity + |
>100mg Human IgG /mL packed resin |
Max.pressure |
10MPa |
Salt Tolerance |
0-5M,all common salts |
Chemical Stability |
All common solutions: water ,1M NaOH,8M Urea,6M GuHCl,glycol,0-100% ethanol,acetonitrile,2M acetic acid,1M HCl, other common solutions. |
pH stability |
1~14 |
Operating temperature |
Operating temperature:2~30℃, do not freeze. |
Storage |
2~30℃,20% ethanol or 0.1M NaOH |
Avoid |
Avoid exposure to strong oxidants, oxidizing acids, strong reducing agents, acetone or benzyl alcohol. |
+10%DBC,Buffer A:50mM Sodium acetate,pH5.5,Column bed height 10cm,Flow velocity:100cm/h
Product |
Cat. No. |
Pack size |
MegaPoly BXS |
AI05701 |
25mL |
AI05702 |
100mL |
|
AI05703 |
500mL |
|
AI05704 |
1L |
|
AI05705 |
5L |
|
AI05706 |
10L |
|
AI05707 |
20L |
|
AI05708 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast MegaPoly BXS |
EI05721 |
1×1mL |
EI05731 |
5×1mL |
|
EI05723 |
1×5mL |
|
EI05733 |
5×5mL |
|
EzScreen MegaPoly BXS |
EI05725 |
1×4.6mL |
EI05735 |
5×4.6mL |
|
EzSelect MegaPoly BXS |
EI05726 |
8×600μL |
EzLoad 16/10 MegaPoly BXS |
EI05701 |
1 pcs |
EzLoad 26/10 MegaPoly BXS |
EI05711 |
1 pcs |