Octyl Bestarose 4FF-HIC Resins丨Bestchrom

Octyl Bestarose 4FF

Product Characteristics

HIC resin using Octyl as its ligands
Agarose matrix with bead size of 90μm, good non-specific adsorption and good pressure/flow characteristic
Ligands with intermediate hydrophobicity, which is used for the capture and intermediate purification of macro-biomolecules with intermediate hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.

Diamond Octyl Mustang

Octyl Bestarose HP

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Octyl Bestarose 4FF is obtained by coupling Octyl fat chains on agarose base matrix. The high physical and chemical stability of the agarose base matrix allows good pressure flow velocity performance, making it ideal for large-scale purification processes, especially in the capture or intermediate purification stages, where a high flow velocity separation process is required.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose
Functional group	Octyl
Ligand concentration	~5μmol Ligand/mL resin
Particle size⁺	45~165um(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	≥150cm/h(0.1MPa BXK50/60, H=25cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH⁺⁺,1M HAc⁺⁺,1mM HCl,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP), 3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	90~150cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Octyl Bestarose 4FF	AH0051	25mL
	AH102307	100mL
	AH102311	500mL
	AH0053	1L
	AH0054	5L
	AH102314	10L
	AH102315	20L
	AH102316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Octyl 4FF	EH00521	1×1mL
	EH022	5×1mL
	EH202303	1×5mL
	EH023	5×5mL
EzScreen Octyl 4FF	EH00525	1×4.6mL
EzScreen Octyl 4FF	EH00535	5×4.6mL
EzLoad 16/10 Octyl 4FF	EH024	1 pcs
EzLoad 26/10 Octyl 4FF	EH025	1 pcs