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Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
The base matrix of Octyl Bestarose HP hydrophobic resin is highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compounds as well as the good compatibility with biological macromolecules. The average particle size of Octyl Bestarose HP is 34μm, providing higher resolution than Octyl Bestarose FF. The surface contains hydrophobic Octyl ligands, which is particularly suitable for the polishing of recombinant proteins, antibodies, vaccines, VLP particles and other intermediately hydrophobic biological molecules.
|
Appearance |
White slurry |
|
Matrix |
Highly cross-linked agarose |
|
Functional group |
Octyl |
|
Particle size+ |
24~44μm(the average particle size is 34μm) |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity |
≥200cm/h(0.1MPa BXK50/30, H=15cm, 25℃) |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol |
|
pH stability |
2~14(CIP),3~13(working) |
|
Operating temperature |
Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ autoclaving (20min) |
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Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
60~120cm/h |
+Particle size is normally distributed, and particles within this range account for more than 75 % of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Octyl Bestarose HP |
AH0081 |
25mL |
|
AH202107 |
100mL |
|
|
AH202111 |
500mL |
|
|
AH0083 |
1L |
|
|
AH0084 |
5L |
|
|
AH202114 |
10L |
|
|
AH202115 |
20L |
|
|
AH202116 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Octyl HP |
EH00821 |
1×1mL |
|
EH202151 |
5×1mL |
|
|
EH202103 |
1×5mL |
|
|
EH202153 |
5×5mL |
|
|
EzScreen Octyl HP |
EH00825 |
1×4.6mL |
|
EH00835 |
5×4.6mL |
|
|
EzLoad 16/10 Octyl HP |
EH00801 |
1 pcs |
|
EzLoad 26/10 Octyl HP |
EH00811 |
1 pcs |
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