Phenyl Bestarose FF(LS)-HIC resins丨Bestchrom

Phenyl Bestarose FF(LS)

Product Characteristics

HIC resin using Phenyl as its ligands
Different selectivity from that of Phenyl Bestarose FF（HS）
Agarose matrix with bead size of 90μm, good non-specific adsorption and pressure/flow characteristic
Ligands with strong hydrophobicity, suitable for the capture and intermediate purification of macro-biomolecules with weak hydrophobicity such as recombinant protein, antibody, enzyme and nucleic acids.

Phenyl Bestarose FF(HS)

Diamond Phenyl(HS)

Description

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Phenyl Bestarose FF (LS) is formed by coupling low substitution (Low Sub) hydrophobic Phenyl groups on agarose base matrix. The High physical and chemical stability of agarose base matrix make provides it good pressure flow velocity performance, making it an ideal choice for large-scale purification process. Suitable for the separation and purification of recombinant proteins, antibodies, enzymes, nucleic acids and other biomolecules with low hydrophobicity, especially in the capture or intermediate purification stage requiring high flow velocity separation process.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly Cross-linked agarose
Functional group	Phenyl
Ligand concentration	~ 25μmol Ligand/mL resin
Particle size⁺	45~165μm(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	250~400cm/h(0.1MPa BXK50/60, H=25cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 1M NaOH⁺⁺, 1M HAc⁺⁺, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	150~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Phenyl Bestarose FF(LS)	AH0021	25mL
	AH211307	100mL
	AH211311	500mL
	AH0023	1L
	AH0024	5L
	AH0025	10L
	AH0026	20L
	AH211316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Phenyl FF(LS)	EH00221	1×1mL
	EH007	5×1mL
	EH211303	1×5mL
	EH008	5×5mL
EzScreen Phenyl FF(LS)	EH00225	1×4.6mL
EzScreen Phenyl FF(LS)	EH00235	5×4.6mL
EzLoad 16/10 Phenyl FF(LS)	EH009	1 pcs
EzLoad 26/10 Phenyl FF(LS)	EH010	1 pcs