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Phenyl HIC Resins

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Hydrophobic Interaction Resins
Phenyl HIC Resins

Phenyl Bestarose HP

Phenyl Bestarose HP

Product Characteristics

  • HIC resin using Phenyl as its ligands
  • Agarose matrix with bead size of 34μm, good non-specific adsorption and good resolution
  • For polishing of macro-biomolecules with weak hydrophobicity
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Description
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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Phenyl Bestarose HP is based on highly cross-linked agarose, which retains excellent hydrophilicity and pore structure of natural polysaccharide compounds while having good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which enjoys higher resolution efficiency than Phenyl Bestarose FF. The surface contains Phenyl hydrophobic ligands, which are particularly suitable for the polishing and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

2. Technical characteristics

Appearance

White slurry

Matrix

Cross-linked agarose

Particle size+

24~44μm(the average particle size is 34μm)

Functional group

Phenyl

Ligand concentration

~ 25μmol Ligand/mL resin

Dynamic binding capacity

~ 45mg α-Chymotrypsinogen /mL packed resin

Max. pressure

0.3MPa

Pressure flow velocity

≥200cm/h(0.1MPa,BXK 50/30, H=15cm, 25℃)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol

pH stability

3~13(CIP), 3~12(working)

Operating temperature

Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min)

Storage+++

2~30℃, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

60-120cm/h

 

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product

Cat. No.

Pack size

Phenyl Bestarose HP

AH201105

25mL

AH201107

100mL

AH201111

500mL

AH0042

1L

AH0043

5L

AH0044

10L

AH201115

20L

AH201116

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast Phenyl HP

EH00421

1×1mL

EH017

5×1mL

EH201103

1×5mL

EH018

5×5mL

EzScreen Phenyl HP

EH00425

1×4.6mL

EH00435

5×4.6mL

EzLoad 16/10 Phenyl HP

EH019

1 pcs

EzLoad 26/10 Phenyl HP

EH020

1 pcs

 

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