Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Phenyl Bestarose HP is based on highly cross-linked agarose, which retains excellent hydrophilicity and pore structure of natural polysaccharide compounds while having good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which enjoys higher resolution efficiency than Phenyl Bestarose FF. The surface contains Phenyl hydrophobic ligands, which are particularly suitable for the polishing and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.
Appearance |
White slurry |
Matrix |
Cross-linked agarose |
Particle size+ |
24~44μm(the average particle size is 34μm) |
Functional group |
Phenyl |
Ligand concentration |
~ 25μmol Ligand/mL resin |
Dynamic binding capacity |
~ 45mg α-Chymotrypsinogen /mL packed resin |
Max. pressure |
0.3MPa |
Pressure flow velocity |
≥200cm/h(0.1MPa,BXK 50/30, H=15cm, 25℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol |
pH stability |
3~13(CIP), 3~12(working) |
Operating temperature |
Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min) |
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
60-120cm/h |
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Phenyl Bestarose HP |
AH201105 |
25mL |
AH201107 |
100mL |
|
AH201111 |
500mL |
|
AH0042 |
1L |
|
AH0043 |
5L |
|
AH0044 |
10L |
|
AH201115 |
20L |
|
AH201116 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Phenyl HP |
EH00421 |
1×1mL |
EH017 |
5×1mL |
|
EH201103 |
1×5mL |
|
EH018 |
5×5mL |
|
EzScreen Phenyl HP |
EH00425 |
1×4.6mL |
EH00435 |
5×4.6mL |
|
EzLoad 16/10 Phenyl HP |
EH019 |
1 pcs |
EzLoad 26/10 Phenyl HP |
EH020 |
1 pcs |