Protein G Bestarose 4FF Affinity Chromatography Resin

Protein G Bestarose 4FF

Product Characteristics

Protein G Bestarose 4FF is often used for separation Purification of antibodies or antibody fragments from cell culture media and purification of antibodies from sera of various species.
The high alkali tolerance of protein ligands can withstand CIP of 0.5~1.0 M NaOH during the cleaning process.
High binding capacity, can reduce production costs and improve production efficiency.

IgM Capture Bestarose FF

Novo-A Diamond

Description

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1. Introduction

Protein G Bestarose 4FF is an affinity resin made by fixing Protein G to Bestarose 4FF. Protein G has a broader binding spectrum than Protein A. At the same time, Protein G has strong binding to the Fc fragment of the antibody, while having weak interaction with the Fab fragment of the antibody, so Protein G Bestarose 4FF is often used for separation Purification of antibodies or antibody fragments from cell culture media and purification of antibodies from sera of various species.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose, 4%
Particle size⁺	45-165μm
Functional group	Protein G (E.coli)
Ligand concentration	~2mg Protein G /mL resin
Cross-linking method	Cyanogen bromide activation
Dynamic binding capacity⁺⁺	~20mg human IgG /mL packed resin
Chemical stability	40℃,1 week: 1M HAc (pH2.0),20mM sodium phosphate, 1%SDS, 6M GuHCl, 70% ethanol At room temperature 2h: 0.1 M HCl (pH1.0), 8M Urea (pH10.5)
Physical stability	Volume changes due to changes in pH or ionic strength are negligible
Max. pressure	0.3MPa
Pressure flow velocity	150~250cm/h (0.1MPa BXK50, H=25cm)
pH stability	3~9(working), 2~10 (CIP)
Storage⁺⁺⁺	2~8℃,20% ethanol or 2% benzyl alcohol

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.

++ Linear flow velocity 200cm / h, bed height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH 7.4

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Protein G Bestarose 4FF	AA0142	25mL
	AA104307	100mL
	AA104311	500mL
	AA0144	1L
	AA0145	5L
	AA104314	10L

Prepacked columns	Cat. No.	Pack size
EzFast Protein G 4FF	EA104301	1×1mL
	EA031	1×5mL
	EA032	5×1mL
	EA033	5×5mL
EzScreen Protein G 4FF	EA01425	1×4.6mL
EzScreen Protein G 4FF	EA01435	5×4.6mL
EzLoad 16/10 Protein G 4FF	EA104304	1 pcs
EzLoad 26/10 Protein G 4FF	EA104306	1 pcs