Analytical Products

1. Product Introduction

Protein L can bind variable light chain (VL) of Kappa variants without interfering the antigen binding sites, which enables its applications in the capture of antibody and antibody fragments in chromatography process. Impacted by solutions and pH variation, Protein L ligands leakage will inevitably occur in chromatography process, leading to Protein L residue in antibody-based drugs. Bestchrom Protein L ELISA Kit is specially designed for the detection of Protein L ligands leakage in a highly sensitive manner, providing precise recognition towards Bestchrom Protein L Diamond resins ligands and therefore, enabling accurate/sensitive measurement of Protein L ligand leakage on various biologics using Protein L Diamond in purification. 

2. Detection Principle

Protein L ELISA Kit can detect Protein L residue from samples via Two-site Sandwich ELISA approach. First dilute Protein L-containing sample with Sample Diluent. Isolate Protein L and sample antibody by boiling. After centrifugation, add sample to and let it react with the polycolonal anti-Protein L pre-coated ELISA Microplate. A secondary anti-Protein L antibody labeled directly with horse radish peroxidase (HRP) enzyme is simultaneously reacted forming as sandwich complex of solid phase antibody-Protein L-enzyme-linked antibody. After two wash steps to remove unbound reactants, the strips will then react with tetramethyl benzidine (TMB) substrate, producing a color change (from colorless to blue). It will finally turn to yellow after adding Stop Solution. The more Protein L ligands residue exist in sample, the darker the color will be.The Detect optical density (OD) at 450nm and 650nm. OD shall be positively related to Protein L contents in sample.

3. Kit Components

4. Storage

Preserved at 2-8℃.

5. Ordering Information