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Strong Anion Exchange Resins

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Chromatography Resins
Ion Exchange Resins
Strong Anion Exchange Resins

Q Bestarose XL

Q Bestarose XL

Product Characteristics

  • AEX resin with high binding capacity and high flow rate
  • Suitable for the rapid and efficient capture of sample
  • Low non-specific adsorption, good mechanical and chemical stability, long working life.
  • Rapid and efficient processing of large feedstock volume
  • Widely applicable in the purification of macro-biomolecules including proteins and nucleic acid in bio-pharmacueticals and bio-engineering
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

Bestarose XL series resin is obtained by coupling linear dextran molecules on highly cross-linked agarose matrix, before coupling various functional groups to dextran molecules. This structure reduces the steric hindrance effect among biological molecules, greatly improving the binding of the target molecule.  

Q Bestarose XL is a strong anion exchange resin, which can directly capture biological macromolecules such as viruses from the feed stock. It enjoys high binding capacity and easy scale-up for industrial processing.

 

2.Technical characteristics

Appearance

White slurry

Matrix

highly cross-linked agarose with dextran chain

Functional group

Quaternary amine group

Particle size+

45~165μm

Ionic capacity

180~260μmol Cl-/mL packed resin

Dynamic binding capacity++

> 160 mg BSA/mL resin

Max. pressure

0.3MPa

Pressure flow velocity+++

≥700cm/h (0.1MPa, BXK 50/30 ,H=15cm, 20℃)

Chemical stability

Stable in common aqueous buffers: 1M NaOH++++, 6M GuHcl, 30% isopropyl alcohol, 20% ethanol  

Avoid contact with oxidizing agents.

pH stability

2~14(CIP), 2~12(working)

Operating temperature

Operating temperature:2~40℃, do not freeze. Can tolerate 121℃ autoclaving (20min) .

Storage+++++

2~30℃, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

300~500cm/h

 

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load under 50mM Tris+1M NaCl pH 7.5, flow velocity of 2.2mL /min.  

+++The pressure flow velocity is linear flow velocity of BXK50/30 column, column bed height 15cm, 20℃ 0.1MPa pressure. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, ++and the pressure given should not be regarded as the maximum tolerated pressure of the resin. Pressure flow velocity is shown in Fig 1.

++++1M NaOH only be used for cleaning.

+++++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

Q Bestarose XL

AI0061

25mL

AI0062

100mL

AI0063

500mL

AI0064

1L

AI0065

5L

AI0066

10L

AI0067

20L

AI214316

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast Q XL

EI00621

1×1mL

EI214451

5×1mL

EI214403

1×5mL

EI214453

5×5mL

EzScreen Q XL

EI00625

1×4.6mL

EI00635

5×4.6mL

EzLoad 16/10 Q XL

EI029

1 pcs

EzLoad 26/10 Q XL

EI030

1 pcs

 

 

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