Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Bestarose XL series resin is obtained by coupling linear dextran molecules on highly cross-linked agarose matrix, before coupling various functional groups to dextran molecules. This structure reduces the steric hindrance effect among biological molecules, greatly improving the binding of the target molecule.
Q Bestarose XL is a strong anion exchange resin, which can directly capture biological macromolecules such as viruses from the feed stock. It enjoys high binding capacity and easy scale-up for industrial processing.
Appearance |
White slurry |
Matrix |
highly cross-linked agarose with dextran chain |
Functional group |
Quaternary amine group |
Particle size+ |
45~165μm |
Ionic capacity |
180~260μmol Cl-/mL packed resin |
Dynamic binding capacity++ |
> 160 mg BSA/mL resin |
Max. pressure |
0.3MPa |
Pressure flow velocity+++ |
≥700cm/h (0.1MPa, BXK 50/30 ,H=15cm, 20℃) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH++++, 6M GuHcl, 30% isopropyl alcohol, 20% ethanol Avoid contact with oxidizing agents. |
pH stability |
2~14(CIP), 2~12(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. Can tolerate 121℃ autoclaving (20min) . |
Storage+++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommended flow velocity |
300~500cm/h |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load under 50mM Tris+1M NaCl pH 7.5, flow velocity of 2.2mL /min.
+++The pressure flow velocity is linear flow velocity of BXK50/30 column, column bed height 15cm, 20℃ 0.1MPa pressure. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, ++and the pressure given should not be regarded as the maximum tolerated pressure of the resin. Pressure flow velocity is shown in Fig 1.
++++1M NaOH only be used for cleaning.
+++++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Q Bestarose XL |
AI0061 |
25mL |
AI0062 |
100mL |
|
AI0063 |
500mL |
|
AI0064 |
1L |
|
AI0065 |
5L |
|
AI0066 |
10L |
|
AI0067 |
20L |
|
AI214316 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Q XL |
EI00621 |
1×1mL |
EI214451 |
5×1mL |
|
EI214403 |
1×5mL |
|
EI214453 |
5×5mL |
|
EzScreen Q XL |
EI00625 |
1×4.6mL |
EI00635 |
5×4.6mL |
|
EzLoad 16/10 Q XL |
EI029 |
1 pcs |
EzLoad 26/10 Q XL |
EI030 |
1 pcs |