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Strong Cation Exchange Resins

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Ion Exchange Resins
Strong Cation Exchange Resins

SP Bestarose BB

SP Bestarose BB

Product Characteristics

  • CEX resin with big beads(100~300μm in particle size), for the rapid and efficient processing of large feedstock volume
  • Enables high flow rate and easy scale-up
  • Good chemical stability, enables the use of various solutions in CIP
  • Widely used in the initial capture stage of highly viscous feedstock purification. For example, the extraction of proteins from milk.
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) has an opposite charge to the functional group and can be reversibly bound to the functional group.

Big Beads (BB) resin is based on highly cross-linked big beads agarose base frame. The large particle can tolerate samples with poor clarity, reducing the possibility of column blockage. Besides, its fast flow velocity is suitable for processing large samples in a short time.

SP Bestarose BB is a strong cation exchange resin that can capture biological macromolecules directly from the feed stock. Its high binding capacity and fast flow velocity make it suitable for large-scale production applications.

 

2. Technical characteristics 

Appearance

White slurry

Matrix

Cross-linked agarose, 6%

Functional group

Sulfopropyl

Particle size+

100~300μm

Ionic capacity

180~250μmol H+/mL packed resin

The exclusion limit, Mr

~4× 106 (Globular protein) 

Max. pressure

0.3MPa

Pressure flow velocity++

≥1200cm/h(0.1MPa BXK50/30, H=15cm, 20℃, 0.1M NaCl)

Chemical stability

Stable in common aqueous buffers: 1M NaOH+++,6M GuHCl,30% isopropyl alcohol,70% ethanol

Avoid contact with oxidizing agents

pH stability

3~14(CIP), 4~13 (working)

Operating temperature

Operating temperature:2~40℃, do not freeze.

Storage++++

2~30℃, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc

Recommended flow velocity

200~600cm/h

 

+Particle size is normally distributed, and particles within this range account for more than 80% of the total l.

++ The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.

+++1M NaOH only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

9. Order information

Product

Cat. No.

Pack size

SP Bestarose BB

AI0241

25mL

AI203407

100mL

AI0242

500mL

AI0243

1L

AI0244

5L

AI0245

10L

AI0246

20L

AI203416

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast SP BB

EI02421

1×1mL

EI203451

5×1mL

EI203403

1×5mL

EI203453

5×5mL

EzScreen SP BB

EI02425

1×4.6mL

EI02435

5×4.6mL

EzLoad 16/10 SP BB

EI203404

1 pcs

EzLoad 26/10 SP BB

EI203406

1 pcs

 

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