Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin. (3)An ion (called an equilibrium ion) has an opposite charge to the functional group and can be reversibly bound to the functional group.
Big Beads (BB) resin is based on highly cross-linked big beads agarose base frame. The large particle can tolerate samples with poor clarity, reducing the possibility of column blockage. Besides, its fast flow velocity is suitable for processing large samples in a short time.
SP Bestarose BB is a strong cation exchange resin that can capture biological macromolecules directly from the feed stock. Its high binding capacity and fast flow velocity make it suitable for large-scale production applications.
Appearance |
White slurry |
Matrix |
Cross-linked agarose, 6% |
Functional group |
Sulfopropyl |
Particle size+ |
100~300μm |
Ionic capacity |
180~250μmol H+/mL packed resin |
The exclusion limit, Mr |
~4× 106 (Globular protein) |
Max. pressure |
0.3MPa |
Pressure flow velocity++ |
≥1200cm/h(0.1MPa BXK50/30, H=15cm, 20℃, 0.1M NaCl) |
Chemical stability |
Stable in common aqueous buffers: 1M NaOH+++,6M GuHCl,30% isopropyl alcohol,70% ethanol Avoid contact with oxidizing agents |
pH stability |
3~14(CIP), 4~13 (working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage++++ |
2~30℃, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc |
Recommended flow velocity |
200~600cm/h |
+Particle size is normally distributed, and particles within this range account for more than 80% of the total l.
++ The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
SP Bestarose BB |
AI0241 |
25mL |
AI203407 |
100mL |
|
AI0242 |
500mL |
|
AI0243 |
1L |
|
AI0244 |
5L |
|
AI0245 |
10L |
|
AI0246 |
20L |
|
AI203416 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast SP BB |
EI02421 |
1×1mL |
EI203451 |
5×1mL |
|
EI203403 |
1×5mL |
|
EI203453 |
5×5mL |
|
EzScreen SP BB |
EI02425 |
1×4.6mL |
EI02435 |
5×4.6mL |
|
EzLoad 16/10 SP BB |
EI203404 |
1 pcs |
EzLoad 26/10 SP BB |
EI203406 |
1 pcs |