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Strong Cation Exchange Resins

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Ion Exchange Resins
Strong Cation Exchange Resins

SP Bestarose FF

SP Bestarose FF

Product Characteristics

  • Strong CEX resin based on agarose matrix
  • Suitable for the rapid and efficient capture of sample
  • Low non-specific adsorption, good mechanical and chemical stability, long working life
  • Widely applicable in the purification of biomolecules including recombinant proteins and insulins in bio-pharmaceuticals and bio-engineering.
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

SP Bestarose FF (Fast Flow) is a strong cation exchanger formed by coupling sulfopropyl onto high flow velocity agarose matrix.

 

2. Technical characteristics

Appearance

White slurry

Matrix

Cross-linked agarose, 6%

Functional group

Sulfopropyl

Particle size+

45~165μm

Ionic capacity

180-250μmol/ mL packed resin

Max. pressure

0.3MPa

Pressure flow velocity++

≥400cm/h(0.1MPa,BXK50/30,H=15cm,20℃,0.1M NaCl)

Chemical Stability

Stable in common aqueous buffers:1M NaOH+++,1M HAC+++,10mM NaOH, 6M GuHCl,8M Urea, 70% ethanol ,30% Isopropyl alcohol, 20% ethanol, 2%Benzyl alcohol, 30% acetonitrile

pH stability

3 ~14 (CIP), 4~13(Working)

Operating temperature

Operating temperature:2~40℃, do not freeze.

Storage++++

2~30℃, 20% ethanol +0.2M NaAc or 2% benzyl alcohol +0.2M NaAc

Recommend flow velocity

60-300cm/h

Avoid

Avoid contact with oxidizing agents, cationic detergents. 

Avoid long time exposures (1 week, 20°C) to pH <4.

 

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin. Pressure flow velocity is shown in Fig 1.

+++1M NaOH and 1M HAc only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

SP Bestarose FF

AI0011

25mL

AI0012

100mL

AI0013

500mL

AI0014

1L

AI0015

5L

AI0016

10L

AI0017

20L

AI203316

40L

 

Prepacked columns

Cat. No.

Pack size

EzFast SP FF

EI00121

1×1mL

EI001

5×1mL

EI203303

1×5mL

EI002

5×5mL

EzScreen SP FF

EI00125

1×4.6mL

EI00135

5×4.6mL

EzLoad 16/10 SP FF

EI004

1 pcs

EzLoad 26/10 SP FF

EI005

1 pcs

 

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