English
Request a Quote
Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Bestarose XL series resins are obtained by firstly coupling with linear dextran molecules on a highly cross-linked agarose matrix, before coupling various functional groups on the dextran molecules. This structure reduces the steric hindrance effect among biological molecules, greatly improving the binding of the target molecule.
SP Bestarose XL is a strong cation exchange resin that can directly capture biological macromolecules from the feed stock, which can meet the needs of the downstream separation process and is easy to scale up.
|
Appearance |
White slurry |
|
Matrix |
6% highly cross-linked agarose with dextran chain. |
|
Functional group |
Sulfopropyl |
|
Particle size+ |
45~165μm |
|
Ionic capacity |
180~250μmol H+/mL packed resin |
|
Dynamic binding capacity++ |
≥160 mg lysozyme/mL resin |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity+++ |
≥700cm/h(0.1MPa BXK 50/30, H=15cm, 20℃) |
|
Chemical Stability |
Stable in common aqueous buffers:1M NaOH++++, 6M GuHCl, 30% isopropyl alcohol, 20% ethanol |
|
pH stability |
3~14(CIP), 4~13 (working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
|
Storage+++++ |
2~30℃，20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc |
|
Recommended flow velocity |
300~500cm/h |
|
Avoid |
Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20℃) to pH <4. |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ In EzScreen 4.4mL column with column height of 10cm, 10% lysozyme dynamic binding load at flow velocity of 2.2mL /min at 50mM Gly+1M NaOH pH 9.
+++The pressure flow velocity is linear flow velocity of BXK50/30 column, column bed height 15cm, 20℃ 0.1MPa pressure. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, ++and the pressure given should not be regarded as the maximum tolerated pressure of the resin. Pressure flow velocity is shown in Fig 1.
++++1M NaOH only be used for cleaning.
+++++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
SP Bestarose XL |
AI0051 |
25mL |
|
AI0052 |
100mL |
|
|
AI0053 |
500mL |
|
|
AI0054 |
1L |
|
|
AI0055 |
5L |
|
|
AI0056 |
10L |
|
|
AI213315 |
20L |
|
|
AI213316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast SP XL |
EI00521 |
1×1mL |
|
EI213451 |
5×1mL |
|
|
EI213403 |
1×5mL |
|
|
EI213453 |
5×5mL |
|
|
EzScreen SP XL |
EI00525 |
1×4.6mL |
|
EI00535 |
5×4.6mL |
|
|
EzLoad 16/10 SP XL |
EI024 |
1 pcs |
|
EzLoad 26/10 SP XL |
EI025 |
1 pcs |
.png)
.png)
.png)
.png)
.png)
