Ion exchange (IEX) chromatography is a very effective method for separation and purification of biological molecules. The method mainly relies on the interaction between positive and negative charges, and uses the nature and difference of the charges carried by different biomolecules under specific conditions to separate. It has the characteristics of high load capacity, good resolution, controllable conditions and easy amplification. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
The ion exchange resin is composed of three parts: (1) Cross-linked mesh base frame, which is porous, hydrophilic and chemically stable. (2)The functional group fixed on the base frame, which is a charged group, determines the nature of the ion exchange chromatography resin. (3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
XL Big Beads series resin is obtained by firstly coupling linear dextran molecules on the large particle highly cross-linked agarose matrix, before coupling various functional groups to the dextran molecules. This structure reduces the steric hindrance among biomolecules and can greatly increase the binding of target molecules. The large particle matrix can tolerate samples with poor clarity, reducing possibility of column blockage. Its high flow velocity makes it suitable for processing of large samples in a short time.
SP XL Big Beads is a strong cation exchange resin, which can directly capture biological macromolecules from the feed stock, plus its high binding capacity and fast flow velocity, making it suitable for large-scale production applications.
Appearance |
White slurry |
Matrix |
6% highly cross-linked agarose with dextran chain |
Functional group |
Sulfopropyl |
Particle size + |
100~300μm |
Ionic capacity |
180~250μmol H+/mL packed resin |
Max. pressure |
0.3MPa |
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,8M Urea, 70% ethanol , 30% isopropyl alcohol Avoid contact with oxidizing agents |
pH stability |
3~14 (CIP),4~13(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage+++ |
2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc |
Recommended flow velocity |
300~900cm/h |
+Particle size is normally distributed, and particles within this range account for more than80% of the total.
++1M NaOH only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
SP XL Big Beads |
AI0201 |
25mL |
AI213407 |
100mL |
|
AI0202 |
500mL |
|
AI0203 |
1L |
|
AI0204 |
5L |
|
AI0205 |
10L |
|
AI213415 |
20L |
|
AI213416 |
40L |