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Strong Cation Exchange Resins

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Ion Exchange Resins
Strong Cation Exchange Resins

SP XL Big Beads

SP XL Big Beads

Product Characteristics

  • CEX resin with high binding capacity and large bead size(100~300μm)
  • Rapid, efficient processing of large feedstock volume with high viscosity.
  • Good mechanical and chemical stability, long working life
  • For rapid capture of highly viscous sample on large scale in bio-pharmaceuticals and bio-engineering.
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Description
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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for separation and purification of biological molecules. The method mainly relies on the interaction between positive and negative charges, and uses the nature and difference of the charges carried by different biomolecules under specific conditions to separate. It has the characteristics of high load capacity, good resolution, controllable conditions and easy amplification. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

The ion exchange resin is composed of three parts: (1) Cross-linked mesh base frame, which is porous, hydrophilic and chemically stable. (2)The functional group fixed on the base frame, which is a charged group, determines the nature of the ion exchange chromatography resin. (3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

XL Big Beads series resin is obtained by firstly coupling linear dextran molecules on the large particle highly cross-linked agarose matrix, before coupling various functional groups to the dextran molecules. This structure reduces the steric hindrance among biomolecules and can greatly increase the binding of target molecules. The large particle matrix can tolerate samples with poor clarity, reducing possibility of column blockage. Its high flow velocity makes it suitable for processing of large samples in a short time.

SP XL Big Beads is a strong cation exchange resin, which can directly capture biological macromolecules from the feed stock, plus its high binding capacity and fast flow velocity, making it suitable for large-scale production applications.

 

2. Technical characteristics

Appearance

White slurry

Matrix

6% highly cross-linked agarose with dextran chain

Functional group

Sulfopropyl

Particle size +

100~300μm

Ionic capacity

180~250μmol H+/mL packed resin

Max. pressure

0.3MPa

Chemical Stability

Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,8M Urea, 70% ethanol , 30% isopropyl alcohol

Avoid contact with oxidizing agents

pH stability

3~14 (CIP),4~13(working)

Operating temperature

Operating temperature:2~40℃, do not freeze.

Storage+++

2~30℃, 20% ethanol with 0.2M NaAc or 2% benzyl alcohol with 0.2M NaAc

Recommended flow velocity

300~900cm/h

 

+Particle size is normally distributed, and particles within this range account for more than80% of the total.

++1M NaOH only be used for cleaning.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

 

3. Order information

Product

Cat. No.

Pack size

SP XL Big Beads

AI0201

25mL

AI213407

100mL

AI0202

500mL

AI0203

1L

AI0204

5L

AI0205

10L

AI213415

20L

AI213416

40L

 

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