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In a downstream processing lab, various resins can be found for the purification of different bio-substances. For the resins in the bottles, some of them are bright and clear, some are in white color, which makes a Cibacron Blue resin really stand out and get your attention. In this article, we are going to introduce the special resin---blue resin.
To put it simply, blue resin is an affinity resin using Cibacron Blue 3GA dye molecules as ligands. The natural blue of dye molecules gives the resin its unique blue color, giving its name of ‘Blue resin’.
Cibacron Blue 3GA molecules are anthraquinone derivatives, which have amino, sulfonic and phenyl groups as well as nucleotide specificity, ions and hydrophobic capture proteins in the structure of molecules[1]. Generally speaking, blue resin can specifically bind interferons and other plasma proteins in addition to albumins. Besides, it enjoys excellent binding capacity towards various proteases including dehydrogenase, kinase, transferase as well as most adenylate-containing coenzymes including NAD+.
Bestchrom offers 4 types of blue resins, namely, Blue Bestarose FF, Blue Bestarose HP, Diamond Blue and Diamond Blue Mustang. Refer to the following table for technical parameters of resin.
| Resin | Blue Bestarose FF | Blue Bestarose HP | Diamond Blue | Diamond Blue Mustang |
|---|---|---|---|---|
| Ligands | Cibacron Blue 3GA | |||
| Ligand concentration | ~7.3μmol chromophore /mL resin | ~13μmol chromophore /mL resin | ||
| DBC++ | >18mg HSA/ mL resin | ≥24mg HSA/mL resin | ||
| Back pressure | 0.3MPa | 0.5MPa | ||
| Particle size | 90μm | 34μm | 75μm | 36~44μm |
| pH stability |
4~12（operation） 3~13(CIP) |
4~12（operation） 3~13(CIP) |
2~13（operation） 2~13(CIP) |
2~13（operation） 2~13(CIP) |
++Residence time: 4min
Sample: human serum albumin
Column: EzFast ，1 mL
Flow: 0.2 mL/min
Binding buffer: 50 mM KH2PO4 ，pH7.0
Elution buffer: 1.5M NaCl，50 mM KH2PO4 ，pH7.0
Fig1. Chromatogram of Blue Bestarose FF
Sample: human blood supernatant diluted sample
Column: EzFast Blue FF 5 mL
Flow: 1 mL/min
Equilibration: 20 Mm NaH2PO4 ，pH7.0
Wash: 20 mM NaH2PO4 ，0.5M NaCl，pH7.0
Elution: 20 mM NaH2PO4，2 M NaCl，pH7. 0
Fig.2. SDS-PAGE analysis of eluates for EzFast Blue FF [2]
| Sample |
Concentration (mg/mL) |
Volume (mL) |
Purity (%) |
Total (mg) |
Recovery (%) |
|---|---|---|---|---|---|
| human blood supernatant diluted sample | 15.15 | 120.00 | 41.0 | 745.38 | 100.00 |
| Affinity chromatography flow-through peak | 9.50 | 130.00 | 1.1 | 13.59 | 1.82 |
| Affinity chromatography impurity peak | 13.30 | 30.00 | 77.0 | 307.23 | 41.22 |
| Affinity chromatography target molecules peak | 12.08 | 34.00 | 90.0 | 369.65 | 49.59 |
Purity of eluate can be 90%, recovery rate is 49. 59%, concentration is 12. 08 mg/mL.
| Resin | Cat.No | Specification |
|---|---|---|
| Blue Bestarose FF | AA210305 | 25mL |
| AA210307 | 100mL | |
| AA0092 | 500 mL | |
| AA0093 | 1L | |
| AA0094 | 5L | |
| AA0095 | 10L | |
| Blue Bestarose HP | AA0282 | 25mL |
| AA0283 | 100mL | |
| AA0284 | 500 mL | |
| AA0285 | 1L | |
| AA0286 | 5L | |
| AA210114 | 10L | |
| Diamond Blue | AA310305 | 25mL |
| AA310307 | 100mL | |
| AA310311 | 500 mL | |
| AA310312 | 1L | |
| AA310313 | 5L | |
| AA310314 | 10L | |
| Diamond Blue Mustang | AA310105 | 25mL |
| AA310107 | 100mL | |
| AA310111 | 500mL | |
| AA310112 | 1L | |
| AA310113 | 5L | |
| AA310114 | 10L |
Call Bestchrom sales team and technical support team in your area: 400-820-5172
[1] Subramanian S. Dye-ligand affinity chromatography: the interaction of Cibacron Blue F3GA with proteins and enzymes. CRC Crit Rev Biochem. 1984;16(2):169-205
[2] Lv Xingkai, WANG Yin, REN Keyun, HU Shangjie, HE Kun, Chen Aifang, Zhang Fan. Analysis of the purification process of human blood albumin by capillary electrophoresis [J]. Chinese Journal of Biologics, 2020, 33 (12): 1441-1444
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