Analytical Products

1. Product Introduction

BVH ELISA Kit is a type of Sandwich ELISA kit, which is used for the detection of residual BVH ligand leakage in pharmaceutical products after using BVH Diamond resin in purification process.  

Kit characteristics:

·High detection sensitivity, standard curve OD ratio of 0pg/mL and 160pg /mL is approximately 2 to 3 times.

·High precision, inter-and intra-plate variability is <10%.

·Acid-treatment for Sample pre-treatment, eliminate sample boiling and the subsequent centrifugation steps. Incubation step is carried out at the same time of antibody/antigen capture and antibody detection. Eliminate inconvenience of multiple plate washing. Simplify operation. 

2. Detection Principle

BVH ELISA Kit can detect BVH residue from samples via Two-site Sandwich ELISA approach. First dilute BVH-containing sample with Sample Diluent (provided in the Kit). Add denaturing Buffer and mix to isolate BVH and sample antibody. Add denatured sample to and let it react with the polycolonal anti-BVH pre-coated ELISA Microplate. A second anti-BVH antibody labeled directly with horse radish peroxidase (HRP) enzyme is simultaneously reacted forming as sandwich complex of solid phase antibody-BVH-enzyme-linked antibody. After a wash step to remove any unbound reactants, the strips are then react with tetramethyl benzidine (TMB) substrate, producing a color change (from colorless to blue). It will finally turn to yellow after using Stop Solution. The more BVH ligands residue is in sample, the darker the color will be.The Detect optical density (OD) at 450nm and 650nm. OD shall be positively related to BVH contents in sample.

3. Kit Components

4. Storage

Kit: Preserved at 2-8℃.

5. Ordering Information