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Benzamidine Bestarose 4FF(LS) is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 4FF agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc.) and can be used as ligands for purifying such substances.
|
Appearance |
White slurry |
|
Matrix |
Cross-linked agarose, 4% |
|
Average particle size |
~90μm |
|
Functional group |
P-aminobenzidine |
|
Dynamic binding capacity |
20~26mg Trypsin/mL packed resin |
|
Max. pressure |
0.3MPa |
|
Pressure flow velocity |
＞150cm/h,0.1MPa BXK50/30, H=25cm |
|
Chemical stability |
Stable in all common aqueous buffers. |
|
pH stability |
2~8(working),1~9(CIP) |
|
Storage+ |
2~8℃,20% ethanol with 50mM NaAc(pH4.0) or 2% benzyl alcohol with 50mM NaAc(pH4.0) |
|
Recommended flow velocity |
30~300cm/h |
+2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Benzamidine Bestarose 4FF(LS) |
AA0341 |
25mL |
|
AA0342 |
100mL |
|
|
AA0343 |
500mL |
|
|
AA0344 |
1L |
|
|
AA0345 |
5L |
|
|
AA0346 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Benzamidine 4FF(LS) |
EA03421 |
1×1mL |
|
EA03431 |
5×1mL |
|
|
EA03423 |
1×5mL |
|
|
EA03433 |
5×5mL |
|
|
EzScreen Benzamidine 4FF(LS) |
EA03425 |
1×4.6mL |
|
EA03435 |
5×4.6mL |
|
|
EzLoad 16/10 Benzamidine 4FF(LS) |
EA03401 |
1 pcs |
|
EzLoad 26/10 Benzamidine 4FF(LS) |
EA03411 |
1 pcs |
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