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Benzamidine Bestarose 6B is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 6B agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (including trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for the purification of such substances.
|
Appearance |
White slurry |
|
Matrix |
Cross-linked agarose, 6% |
|
Particle size+ |
45~165μm |
|
Functional group |
P-aminobenzidine |
|
Ligand concentration |
~7μmol P-aminobenzidine /mL resin |
|
Dynamic binding capacity++ |
≥10mg Trypsin/mL packed resin |
|
Operation flow velocity |
~75cm/h（BXK16/10,H=5cm,25°C） |
|
Chemical stability |
Stable in common aqueous buffers: 6M GuHCl,8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11 |
|
pH stability |
3~11(working),2~13(CIP) |
|
Storage+++ |
2~8℃,20% ethanol + 50mM NaAc(pH4.0) or 2% benzyl alcohol +50mM NaAc(pH 4.0) |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++Binding capacity is tested at 50mM Tris + 0.5M NaCl pH8.0.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Benzamidine Bestarose 6B |
AA511305 |
25mL |
|
AA511307 |
100mL |
|
|
AA511311 |
500mL |
|
|
AA511312 |
1L |
|
|
AA511313 |
5L |
|
|
AA511314 |
10L |
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