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Microcarrier

Bestdex Cell 1

Bestdex Cell 1

Product Characteristics

  • The optimization of particle size and density provides high yield growth conditions for various cells.
  • After swelling, the microcarrier is transparent, which is convenient for the microscopic examination of the attached cells.
  • Microcarrier substrate are biologically inert and can provide a strong but non-rigid substrate for stirred culture of cells.
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1. Introduction 

Microcarrier culture is a technique which enables the high yield culture of anchorage-dependent cells. Bestdex Cell 1 has been developed specifically for growing a wide range of animal cells in volumes ranging from a few milliliters to several thousand liters. The use of Bestdex Cell 1 in a simple suspension culture system provides a yield of millions of cells per milliliter. It changes the available surface area simply by changing the microcarrier concentration. Applications include large-scale production of cells, viruses, and recombinant cell products (eg., interferon, enzymes, nucleic acids, hormones), cell adhesion, differentiation, and cell function. This product is intended for research purposes only and is not intended for clinical or in vitro diagnostic use.
Bestdex Cell 1 is formed by coupling diethylaminoethyl group to Bestdex G-50.In the process of Cell culture media, the microcarriers provide bioinert matrix, and the cells are highly adhered to the surface of the microcarriers. In addition, the density of Bestdex Cell 1 is similar to that of water, which is conducive to the uniform suspension of cells.
Bestdex Cell 1 is suitable for most established Cell lines. It can also be used for the culture of primary cells and normal diploid cell lines.
The main characteristics of this resin include:
The optimization of particle size and density provides high yield growth conditions for various cells.
After swelling, the microcarrier is transparent, which is convenient for the microscopic examination of the attached cells.
Microcarrier substrate are biologically inert and can provide a strong but non-rigid substrate for stirred culture of cells.

2. Technical Characteristics

Matrix

Dextran

Functional group

Diethylaminoethyl

Particle size 

150~250μm

Ionic capacity

1.40-1.60mmol Cl-/g resin

Deposition velocity

~ 90cm/h

Density

~1.03g/mL

Swelling factor

20~25mL

Approx. area

~4400 cm2/g

Approx. no. microcarriers /g dry resin

~4.3×106

 
Dry powder particles refer to the wet gel particle size after swelling with 0.15M NaCl solution.  The volume of particles in this range accounts for more than 80% of the total volume. 
Particle size may vary in different solutions, and this data is the result of swelling in 0.15M NaCl.  

3. Order Information

Product

Code No.

Pack size

Bestdex Cell 1

AI132305

25g

AI132307

100g

AI132311

500g

AI132312

1kg

AI132313

5kg

AI132316

10kg

AI132314

25kg

 

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