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Blue Bestarose HP is an affinity resin based on fine particle agarose and Cibacron Blue 3GA as ligand. It has the characteristics such as high resolution, stable physical and chemical properties, firmly attached ligands (not easy to drop off), long service life, and a wide range of applications. This resin can bind proteins through specific interactions, as well as non-specifically bind proteins through charges and other effects. Blue Bestarose HP has been widely used in the separation and purification of various proteins, including dehydrogenase, kinase, transferase, serum albumin, interferon and plasma protein.
|
Appearance |
Blue slurry |
|
Average particle size+ |
~34μm (24~44μm) |
|
Functional group |
Cibacron Blue 3GA |
|
Ligand concentration |
~7.3μmol chromophore /mL resin |
|
Dynamic binding capacity |
＞18mg HSA/mL packed resin |
|
Max. pressure |
0.3MPa |
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Chemical stability |
Stable in common aqueous buffers:8M urea, 6M GuHCl, 70% ethanol |
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pH stability |
4~12(working),3~13(CIP) |
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Operating temperature |
Operating temperature:2~40℃, do not freeze. |
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Storage++ |
2~8℃, 20% ethanol + 0.1M KH2PO4(pH 8.0)or 2% benzyl alcohol + 0.1M KH2PO4(pH 8.0) |
|
Recommended flow velocity |
＜150cm/h |
+ The average particle size is the cumulative resin particle size of the filler volume distribution.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Blue Bestarose HP |
AA0282 |
25mL |
|
AA0283 |
100mL |
|
|
AA0284 |
500mL |
|
|
AA0285 |
1L |
|
|
AA0286 |
5L |
|
|
AA210114 |
10L |
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Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Blue HP |
EA210101 |
1×1mL |
|
EA210103 |
1×5mL |
|
|
EA210151 |
5×1mL |
|
|
EA210153 |
5×5mL |
|
|
EzScreen Blue HP |
EA02825 |
1×4.6mL |
|
EA02835 |
5×4.6mL |
|
|
EzLoad 16/10 Blue HP |
EA210104 |
1 pcs |
|
EzLoad 26/10 Blue HP |
EA210106 |
1 pcs |
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