Analytical Products

1. Product Introduction

The BVH ELISA Kit is a sandwich-type ELISA detection kit designed to detect ligand contamination in biopharmaceutical samples purified using BVH Diamond affinity chromatography media.

This kit offers the following features:

High sensitivity: the OD ratio between 0 pg/mL and 62.5 pg/mL on the standard curve is approximately 2–3 times.

High precision: both inter-batch and intra-batch variation are < 10%.

Simple sample pretreatment: acid treatment is used, eliminating the need for complicated heating or centrifugation steps. Capture antibody, antigen, and detection antibody can be incubated simultaneously, without multiple washes, making the procedure easy to perform.

2. Detection Principle

This kit is based on a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to measure BVH residues in samples. Samples containing BVH are first diluted with the Sample Diluent provided in the kit, then mixed with Denaturing Buffer to dissociate BVH from the sample antibodies. The denatured sample is then added to a microplate pre-coated with polyclonal anti-BVH capture antibodies. A second anti-BVH detection antibody, directly conjugated to horseradish peroxidase (HRP), forms a solid-phase antibody–BVH–enzyme-labeled antibody sandwich complex. After a single wash step to remove all unbound reactants, the complex reacts with tetramethylbenzidine (TMB), changing color from colorless to blue, and finally turning yellow upon addition of Stop Solution. The color intensity is proportional to the amount of BVH ligand present in the sample. Absorbance (OD value) is measured at 450 nm and 650 nm, and the OD value is positively correlated with the BVH content in the sample.

3. Kit Components

4. Storage

The kit should be stored at 2–8°C.

5. Ordering Information