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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecules. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high binding capacity, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
However, when doing ion exchange chromatography, the salt content in the sample should not be too high. The mixed-mode ion exchange resin has a certain salt tolerance by introducing benzene ring to the ligand, which eliminates the desalting step of high salinity samples and expands the application of ion exchange as a result.
Based on fine beads made from highly rigid agarose, Diamond MIX-A is mixed-mode strong anion exchange resin, functioning in both ionic and hydrophobic interactions.
|
Appearance |
White slurry |
|
Matrix |
High rigidity agarose |
|
Functional group |
Mixed-mode strong anion group |
|
Average particle size + |
~75μm |
|
Ionic capacity |
90~120μmol Cl-/mL packed resin |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity++ |
≥1200cm/h（0.5MPa ,BXK 100/500, H=20 cm, 20℃） |
|
Chemical stability |
Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,10mM NaOH, 6M GuHCl,8M Urea, 70% ethanol , 30% isopropyl alcohol, 20% ethanol, 2% benzyl alcohol Avoid contact with oxidizing agents, cationic detergents. |
|
pH stability |
2~14(CIP),3~12(working) |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze |
|
Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommended flow velocity |
90-500cm/h |
+The average particle size is the cumulative resin particle size of the filler volume distribution.
++The flow velocity is linear flow velocity of BXK100/500 column, column bed height 20cm, 20℃ 0.5MPa pressure.
+++1M NaOH and 1M HAc only be used for cleaning.
++++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Diamond MIX-A |
AI0101 |
25mL |
|
AI0102 |
100mL |
|
|
AI306311 |
500mL |
|
|
AI0103 |
1L |
|
|
AI0104 |
5L |
|
|
AI0105 |
10L |
|
|
AI306315 |
20L |
|
|
AI306316 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond MIX-A |
EI01021 |
1×1mL |
|
EI316351 |
5×1mL |
|
|
EI316303 |
1×5mL |
|
|
EI316353 |
5×5mL |
|
|
EzScreen Diamond MIX-A |
EI01025 |
1×4.6mL |
|
EI01035 |
5×4.6mL |
|
|
EzLoad 16/10 Diamond MIX-A |
EI049 |
1 pcs |
|
EzLoad 26/10 Diamond MIX-A |
EI050 |
1 pcs |
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