Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecules. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high binding capacity, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
However, when doing ion exchange chromatography, the salt content in the sample should not be too high. The mixed-mode ion exchange resin has a certain salt tolerance by introducing benzene ring to the ligand, which eliminates the desalting step of high salinity samples and expands the application of ion exchange as a result.
Based on fine beads made from highly rigid agarose, Diamond MIX-A Mustang is mixed-mode strong anion exchange resin, functioning in both ionic and hydrophobic interactions.
Appearance |
White slurry |
Matrix |
High rigidity agarose |
Functional group |
Mixed-mode strong anion group |
Average particle size+ |
36~44μm |
Ionic capacity |
80~110μmol Cl-/mL packed resin |
Max. pressure |
0.5MPa |
Pressure flow velocity |
≥450cm/h(0.5MPa, BXK 100/500, H=20cm, 20℃) |
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH++,1M HAc++, 2M NaCl, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents. |
pH stability |
2~14 (CIP), 3~12(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze |
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommend flow velocity |
100-300cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond MIX-A Mustang |
AI0151 |
25mL |
AI0152 |
100mL |
|
AI306211 |
500mL |
|
AI0153 |
1L |
|
AI0154 |
5L |
|
AI0155 |
10L |
|
AI306215 |
20L |
|
AI306216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast MIX-A Mustang |
EI01521 |
1×1mL |
EI306251 |
5×1mL |
|
EI306203 |
1×5mL |
|
EI306253 |
5×5mL |
|
EzScreen MIX-A Mustang |
EI01525 |
1×4.6mL |
EI01535 |
5×4.6mL |
|
EzLoad 16/10 MIX-A Mustang |
EI306204 |
1 pcs |
EzLoad 26/10 MIX-A Mustang |
EI306206 |
1 pcs |