Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields. However, when doing ion exchange chromatography, it is required that the salt content in the sample should not be too high. By introducing benzene ring into the ligand, the compound ion exchange resin has a certain tolerance to salt, which avoids the desalting step of high-salt samples and expands the application range of ion exchange.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. The Diamond substrate is a highly rigid agarose substrate, which is formed by chemical modification and modification of the traditional Bestarose 6FF substrate with better mechanical properties. (2)Functional group fixed on the substrate, which is a compound charged group, usually consisting of a charged part and a hydrophobic part. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond MMC is a mixed-mode weak cation exchange resin formed by coupling compound groups with benzene ring and carboxyl group on highly rigid agarose microspheres.
Appearance |
White slurry |
Matrix |
High rigidity agarose |
Functional group |
Complex weak cationic group |
Average particle size+ |
~75μm |
Ionic capacity |
70~90μmol H+/mL packed resin |
Dynamic binding capacity ++ |
> 28 mg BSA/mL packed resin (30mS/cm) |
Max. pressure |
0.5MPa |
Pressure flow velocity+++ |
≥1200cm/h(0.5MPa BXK 100/500 , H=20cm,20℃) |
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH++++,1M HAC++++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol, 20% ethanol, 2% benzyl alcohol Avoid contact with oxidizing agents, cationic detergents. |
pH stability |
3~14(CIP),3~12(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage+++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol. |
Recommend flow velocity |
90-500cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++ BXH5/100 column with column height of 10cm, 10%BSA dynamic binding load under the condition of 50mM NaAc+ 0.25m NaCl pH 4.75 and flow velocity of 2mL /min,
+++The flow velocity is a linear flow velocity with BXK100/500 20cm column height at 20℃ and pressure of 0.5MPa.
++++1M NaOH and 1M HAc only be used for cleaning.
+++++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Diamond MMC |
AI0091 |
25mL |
AI0092 |
100mL |
|
AI305311 |
500mL |
|
AI0093 |
1L |
|
AI0094 |
5L |
|
AI0095 |
10L |
|
AI305315 |
20L |
|
AI305316 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Diamond MMC |
EI00921 |
1×1mL |
EI315351 |
5×1mL |
|
EI315303 |
1×5mL |
|
EI315353 |
5×5mL |
|
EzScreen Diamond MMC |
EI00925 |
1×4.6mL |
EI00935 |
5×4.6mL |
|
EzLoad 16/10 Diamond MMC |
EI044 |
1 pcs |
EzLoad 26/10 Diamond MMC |
EI045 |
1 pcs |