Heparin Bestarose HP (High Performance) is a family-specific affinity chromatography resin made by covalently coupling heparin to fine particles with high cross-linking agarose. Widely used in the separation and purification of various biological molecules, especially various enzymes, including antithrombin III, thrombin-like, human coagulation factor IX, XI, VIII, lipoprotein lipase, collagenase, DNA polymerase. It can also bind human interleukin, human prostate growth factor, recombinant human vascular endothelial growth factor, cartilage growth factor, basic fibroblast growth factor, recombinant human acid fibroblast growth factor, recombinant hepatocyte growth factor, and recombinant murine heparin cofactor II, recombinant human platelet fourth factor, recombinant human endostatin, recombinant human keratinocyte growth factor and other biological macromolecules. The resin has the characteristics such as high resolution, physical and chemical stability, firmly attached ligands, long service life, and a wide range of applications.
Appearance |
White slurry |
Matrix |
Highly cross-linked agarose |
Particle size+ |
24~44μm (the average particle size is 34μm) |
Functional group |
Heparin |
Ligand concentration |
~10mg Heparin Ligand/mL resin |
Dynamic binding capacity |
2-5 mg bovine AT III/ mL packed resin |
Chemical stability |
Stable in common aqueous buffers: 8M urea, 6M GuHCl, 70% ethanol, 50mM sodium acetate(pH4), 10% glycerin, 0.1M NaOH (20℃, a week) |
pH stability |
5~10 |
Storage++ |
2~30℃, 20% ethanol + 50mM NaAc or 2% benzyl alcohol +50mM NaAc |
Recommended flow velocity |
<150cm/h |
+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Heparin Bestarose HP |
AA0261 |
25mL |
AA0262 |
100mL |
|
AA0263 |
500mL |
|
AA0264 |
1L |
|
AA0265 |
5L |
|
AA212114 |
10L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Heparin HP |
EA212101 |
1×1mL |
EA050 |
1×5mL |
|
EA049 |
5×1mL |
|
EA051 |
5×5mL |
|
EzScreen Heparin HP |
EA02625 |
1×4.6mL |
EA02635 |
5×4.6mL |
|
EzLoad 16/10 Heparin HP |
EA212104 |
1 pcs |
EzLoad 26/10 Heparin HP |
EA212106 |
1 pcs |