IMAC Bestarose FF Affinity Chromatography Resin

IMAC Bestarose FF

Product Characteristics

It can chelate the four price points of metal ions, make the chelated metal ions more stable, tolerate higher reducing agents, have good physical and chemical stability, and have advantages in good specificity and fast flow velocity.

Ni Bestarose FF

Ni Bestarose HP

Description

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1. Introduction

IMAC Bestarose FF (Fast flow) is a metal chelating affinity chromatography resin, which can be widely used to separate and purify proteins and peptides. The principle is that the side chains of histidine, cysteine and tryptophan of protein interact with a variety of transition metal ions such as Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, Fe³⁺, so as to achieve the purpose of separation and purification.

IMAC Bestarose FF is crosslinked by cross-linked agarose and aminotriacetic acid (NTA). It can chelate the four price points of metal ions, making the chelated metal ions more stable, to tolerate higher reducing agents, have good physical and chemical stability, as well as advantages in good specificity and fast flow velocity.

2. Technical characteristics

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Particle size⁺	45-165μm
Metal chelating capacity	Ni²⁺/ Zn²⁺:15μmol/mL resin, Cu²⁺:25μmol/mL resin
Dynamic binding capacity	Ni²⁺:~ 40mg His tag protein/mL packed resin
Chemical stability ⁺⁺	1 week at 40℃: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl, 40℃ 12h: 1M NaOH, 70% acetic acid.
pH stability⁺⁺⁺	3~12(working),2~14 (CIP)
Pressure flow velocity	~600cm/h（0.1MPa BXK16/20, H=5cm, 25℃）
Max. pressure	0.3MPa
Storage⁺⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.

++ Stability when removing metal ions.

+++ CIP refers to pH stability when metal ions are removed.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
IMAC Bestarose FF	AA0061	25mL
	AA0062	100mL
	AA207311	500mL
	AA0063	1L
	AA0064	5L
	AA0065	10L
	AA207315	20L

Prepacked columns	Cat. No.	Pack size
EzFast IMAC FF	EA207301	1×1mL
	EA207303	1×5mL
	EA009	5×1mL
	EA010	5×5mL
EzScreen IMAC FF	EA00625	1×4.6mL
EzScreen IMAC FF	EA00635	5×4.6mL
EzLoad 16/10 IMAC FF	EA207304	1 pcs
EzLoad 26/10 IMAC FF	EA207306	1 pcs