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Ni Bestarose FF (Fast Flow) metal chelation chromatography resin is an affinity chromatography resin that pre-chelates metal ion Ni2+ on agarose gel with NTA as ligand. It has the advantages of large adsorption capacity, good selection type, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.
|
Appearance |
Turquoise slurry, can be layered |
|
Matrix |
Highly Cross-linked agarose, 6% |
|
Particle size+ |
45~165μm |
|
Ligand concentration |
12~18μmol Ligand/mL resin |
|
Dynamic binding capacity |
~ 40mg His tag protein/ mL packed resin |
|
Chemical stability ++ |
40℃ One week : 10mM HCl, 0.1M NaOH, 6M GuHCl,8M Urea. 40℃ 12h: 1M NaOH, 70% acetic acid. |
|
pH stability+++ |
3~12 (working) 2~14 (CIP) |
|
Pressure flow velocity |
~600cm/h,BXK16/20,H=5cm,25℃ |
|
Max. pressure |
0.3MPa |
|
Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommend flow velocity |
＜150cm/h |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Ni Bestarose FF |
AA0051 |
25mL |
|
AA0052 |
100mL |
|
|
AA0053 |
500mL |
|
|
AA0054 |
1L |
|
|
AA0055 |
5L |
|
|
AA0056 |
10L |
|
|
AA208315 |
20L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Ni FF |
EA208301 |
1×1mL |
|
EA208303 |
1×5mL |
|
|
EA006 |
5×1mL |
|
|
EA007 |
5×5mL |
|
|
EzScreen Ni FF |
EA00525 |
1×4.6mL |
|
EA00535 |
5×4.6mL |
|
|
EzLoad 16/10 Ni FF |
EA208304 |
1 pcs |
|
EzLoad 26/10 Ni FF |
EA208306 |
1 pcs |
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