IMAC Bestarose HP Affinity Chromatography Resin

IMAC Bestarose HP

Product Characteristics

IMAC Bestarose HP is formed by cross-linking agarose and nitrilotriacetic acid (NTA).
It can chelate the four-valent position of metal ions, make the chelated metal ions more stable, as well as tolerate higher reducing agents.
It has good chemical stability, good specificity and fast flow velocity.

Chelating Bestarose FF

GST Bestarose 4B

Description

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1. Introduction

IMAC Bestarose HP (High Performance) is a high-resolution metal chelating affinity chromatography resin, combined with a variety of transition metal ions such as Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, Fe³⁺, etc, which can be used to separate and purify proteins and peptides. The principle is to use the interaction between the side chains of histidine, cysteine and tryptophan of the protein and metal ions, so as to achieve the purpose of separation and purification.

IMAC Bestarose HP is formed by cross-linking agarose and nitrilotriacetic acid (NTA). It can chelate the four-valent position of metal ions, making the chelated metal ions more stable, as well as tolerate higher reducing agents. It also enjoys good chemical stability, good specificity and fast flow velocity.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose, 6%
Particle size⁺	24~44μm(the average particle size is 34μm)
Chelating ability	~15μmol Ni²⁺/mL resin
Dynamic binding capacity	~ 40mg His tag protein/ mL packed resin(chelating nickel ion)
Chemical stability⁺⁺	1 week at 40℃: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl, 40℃ 12h: 1M NaOH, 70% acetic acid.
Max. pressure	0.3MPa
pH stability⁺⁺⁺	3~12(working) ,2~14(CIP)
Storage⁺⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.

++ Stability when removing metal ions.

+++ CIP refers to pH stability when metal ions are removed.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
IMAC Bestarose HP	AA207105	25mL
	AA207107	100mL
	AA207111	500mL
	AA207112	1L
	AA207113	5L
	AA207114	10L
	AA207115	20L

Prepacked columns	Cat. No.	Pack size
EzFast IMAC HP	EA01021	1×1mL
	EA01031	5×1mL
	EA01023	1×5mL
	EA01033	5×5mL
EzScreen IMAC HP	EA01025	1×4.6mL
EzScreen IMAC HP	EA01035	5×4.6mL
EzLoad 16/10 IMAC HP	EA01001	1 pcs
EzLoad 26/10 IMAC HP	EA01011	1 pcs