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GST Bestarose 4B is an affinity resin made by coupling glutathione to agarose, which is used to specifically purify glutathione S-transferase (GST) and GST fusion protein. GST tag is a tag commonly used in genetic engineering to express fusion proteins, which facilitates soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion protein, this resin can be used to obtain high-purity target protein in one step of purification. The chromatographic resin has mild operating conditions and helps the maintenance of protein activity.
|
Appearance |
White slurry |
|
Matrix |
4% agarose |
|
Particle size+ |
45~165μm |
|
Functional group |
Glutathione with 10 atomic arms |
|
Dynamic binding capacity |
＞5mg GST/mL packed resin |
|
Max. pressure |
0.16 bar |
|
Operation flow velocity |
~75cm/h (BXP16/30 column, H=5cm,25℃) |
|
Chemical stability |
Stable in common aqueous buffers: 1M HAc++, 0.1M NaOH, 70% ethanol, 6M GuHCl (room temperature for 2 hours) |
|
pH stability |
4~13 |
|
Operating temperature |
Operating temperature: 2~30℃, do not freeze |
|
Storage+++ |
2~30℃,20% ethanol or 2% benzyl alcohol |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
GST Bestarose 4B |
AA009305 |
25mL |
|
AA0082 |
100mL |
|
|
AA0083 |
500mL |
|
|
AA0084 |
1L |
|
|
AA0085 |
5L |
|
|
AA009314 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast GST 4B |
EA009301 |
1×1mL |
|
EA009303 |
1×5mL |
|
|
EA015 |
5×1mL |
|
|
EA016 |
5×5mL |
|
|
EzScreen GST 4B |
EA00825 |
1×4.6mL |
|
EzScreen GST 4B |
EA00835 |
5×4.6mL |
|
EzLoad 16/10 GST 4B |
EA009304 |
1 pcs |
|
EzLoad 26/10 GST 4B |
EA009306 |
1 pcs |
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