Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
MMC Bestarose FF (Fast Flow) is a mixed-mode weak cation exchange resin formed by coupling complex groups with benzene ring and carboxyl group on agarose microspheres with high Flow velocity.
Appearance |
White slurry |
Matrix |
Cross-linked agarose, 6% |
Functional group |
Complex weak cationic groups |
Average particle size |
~90μm |
Ionic capacity |
90~120μmol H+/mL packed resin |
Max. pressure |
0.3MPa |
Pressure flow velocity+ |
≥700cm/h(0.1MPa, BXK 50/30, H=15cm, 20℃) |
Chemical Stability |
Stable in common aqueous buffers:1M NaOH++,1M HAC++,6M GuHCl,8M Urea, 70% ethanol ,20% ethanol Avoid contact with oxidizing agents, cationic detergents. |
pH stability |
2~14 (CIP), 4~13(working) |
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
Storage+++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Recommend flow velocity |
60-300cm/h |
+ This flow velocity is a linear flow velocity of BXK50/30 column tube with column bed height of 15cm and pressure of 0.1MPa
at 20℃. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, and the pressure
given should not be regarded as the maximum tolerated pressure of the resin.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
MMC Bestarose FF |
AI0211 |
25mL |
AI0212 |
100mL |
|
AI325211 |
500mL |
|
AI0213 |
1L |
|
AI0214 |
5L |
|
AI325214 |
10L |
|
AI325215 |
20L |
|
AI325216 |
40L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast MMC FF |
EI02121 |
1×1mL |
EI02131 |
5×1mL |
|
EI02123 |
1×5mL |
|
EI02133 |
5×5mL |
|
EzScreen MMC FF |
EI02125 |
1×4.6mL |
EI02135 |
5×4.6mL |
|
EzLoad 16/10 MMC FF |
EI02101 |
1 pcs |
EzLoad 26/10 MMC FF |
EI02111 |
1 pcs |