NHS-activated Bestarose 4FF is a pre-activated agarose resin, the matrix is high-flow agarose Bestarose 4FF, with spacer arms suitable for coupling small molecule ligands.
The pre-activation resin has the following characteristics:
Easy to form amide bond with protein, strong chemical stability.
The extension arm with 10 carbon atoms reduces the steric hindrance of ligand and agarose surface.
Easy operation, avoid using highly toxic raw materials.
Fast flow velocity, suitable for large-scale applications.
Appearance |
White slurry |
Matrix |
Highly cross-linked agarose, 4% |
Particle size+ |
45~165μm |
Activating group |
N-hydroxysuccinimide |
Ligand concentration |
16~23μmol NHS /mL resin |
Coupling functional group |
-NH2 |
Max. pressure |
0.3MPa |
Pressure flow velocity |
~150cm/h(0.1MPa BXK50/60 H=25cm 25°C) |
Chemical stability++ |
Stable in common aqueous buffers: 6M GuHCl,8M Urea, 70% ethanol |
pH stability+++ |
3~13(working), 2~13(CIP) |
Storage |
2~8℃, 100% isopropyl alcohol |
Recommended flow velocity |
150~300cm/h |
+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.
++Data when the coupled ligand is also stable.
+++ No coupling ligand.
Product |
Cat. No. |
Pack size |
NHS-activated Bestarose 4FF |
AA0031 |
25mL |
AA118307 |
100mL |
|
AA0032 |
500mL |