Phenyl Bestarose FF(HS)-HIC resins丨Bestchrom

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1. Introduction

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Phenyl Bestarose FF (HS) is formed by coupling High substitution (High Sub) hydrophobic Phenyl groups on agarose base matrix. The High physical and chemical stability of agarose base matrix provides it with good pressure flow velocity, making it an ideal choice for large-scale purification process. It is suitable for the separation and purification of recombinant proteins, antibodies, enzymes, nucleic acids and other biomolecules with low hydrophobicity, especially in the capture or intermediate purification stage requiring high flow velocity separation process.

2. Technical characteristics

Appearance	White slurry
Matrix	Cross-linked agarose
Functional group	Phenyl
Ligand concentration	~45μmol Ligand/mL resin
Particle size⁺	45~165μm(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	250-400cm/h(0.1MPa BXK50/60, H=25cm, 20℃)
Chemical stability	Stable in common aqueous buffers: 2M NaOH⁺⁺,0.5M NaOH⁺⁺,1M HAc⁺⁺,0.1M HCl, 6M GuHCl,8M Urea, 70% formic acid, 30% isopropyl alcohol, 30% acetonitrile, 2%SDS, 3M(NH₄)₂SO₄, 0.1M KH₂PO₄(PH7.0), 24% ethanol
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (20min)
Storage⁺⁺⁺	2~30℃, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	150-300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M NaOH and 1M HAc only be used for cleaning.

+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
Phenyl Bestarose FF(HS)	AH0031	25mL
	AH221307	100mL
	AH221311	500mL
	AH0033	1L
	AH0034	5L
	AH0035	10L
	AH0036	20L
	AH221316	40L

Prepacked columns	Cat. No.	Pack size
EzFast Phenyl FF(HS)	EH00321	1×1mL
	EH012	5×1mL
	EH221303	1×5mL
	EH013	5×5mL
EzScreen Phenyl FF(HS)	EH00325	1×4.6mL
EzScreen Phenyl FF(HS)	EH00335	5×4.6mL
EzLoad 16/10 Phenyl FF(HS)	EH014	1 pcs
EzLoad 26/10 Phenyl FF(HS)	EH015	1 pcs
EzLoad 16/20 Phenyl FF(HS)	EH031	1 pcs
EzLoad 50/20 Phenyl FF(HS)	EH032	1 pcs