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Plasmid Cap Bestarose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agratose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the binding capacity of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in high salt environment and weakened in low salt environment.
|
Appearance |
White slurry |
|
Matrix |
Highly Cross-linked agarose, 6% |
|
Average particle size |
~34μm |
|
Functional group |
2-mercaptopyridine |
|
Ligand concentration |
~3.5mg 2-mercaptopyridine /mL resin |
|
Dynamic binding capacity |
>2mg Supercoiled plasmid DNA /mL packed resin |
|
Chemical Stability |
Stable in common aqueous buffers: 1M HAC+ ,30% isopropyl alcohol, 70% ethanol , 0.1M NaOH |
|
Max. pressure |
0.3MPa |
|
pH stability |
3~11(Working), 2~13(CIP) |
|
Storage++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Operating temperature |
Operating temperature:15~30℃ |
+1M 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Plasmid Cap Bestarose HP |
AA0201 |
25mL |
|
AA116307 |
100mL |
|
|
AA0203 |
500mL |
|
|
AA0204 |
1L |
|
|
AA0205 |
5L |
|
|
AA116314 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Plasmid HP |
EA216101 |
1×1mL |
|
EA216103 |
1×5mL |
|
|
EA216151 |
5×1mL |
|
|
EA216513 |
5×5mL |
|
|
EzScreen Plasmid HP |
EA02025 |
1×4.6mL |
|
EA02035 |
5×4.6mL |
|
|
EzLoad 16/10 Plasmid HP |
EA216104 |
1 pcs |
|
EzLoad 26/10 Plasmid HP |
EA216106 |
1 pcs |
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