Plasmid Cap Mustang is a thiophilic affinity resin made by fixing the sulfur compound 2-mercaptopyridine to fine particles of high rigidity agarose. The optimized ligand density has appropriate affinity to the super-helix DNA. Its fine particle size boosts the binding capacity of super-helix DNA with big molecular weight. Thiophilic affinity works by using the interaction between electron donor and electron accepter to isolate and purify biomolecules, which is strengthened in high-salt environments and weakened in low-salt environments.
Appearance |
White slurry |
Matrix |
Highly cross-linked agarose |
Average particle size |
36~44μm |
Functional group |
2-mercaptopyridine |
Ligand concentration |
~3.5mg 2- mercaptopyridine /mL resin |
Dynamic binding capacity |
>2mg Supercoiled plasmid DNA /mL packed resin |
Chemical Stability |
Stable in common aqueous buffers: 30% isopropyl alcohol,70% ethanol ,1M HAc+,0.1M NaOH |
Pressure flow velocity |
≥300cm/h(0.1MPa BXK100/500, H=20cm) |
Max. pressure |
0.5MPa |
pH stability |
3~11(working),2~13(CIP) |
Storage++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
Temperature tolerance |
Working temperature:15~30℃ |
+ 1M HAc only be used for cleaning.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
Product |
Cat. No. |
Pack size |
Plasmid Cap Mustang |
AA0211 |
25mL |
AA316207 |
100mL |
|
AA316211 |
500mL |
|
AA0213 |
1L |
|
AA0214 |
5L |
|
AA316214 |
10L |
Prepacked columns |
Cat. No. |
Pack size |
EzFast Plasmid Mustang |
EA316301 |
1×1mL |
EA316303 |
1×5mL |
|
EA316351 |
5×1mL |
|
EA316353 |
5×5mL |
|
EzScreen Plasmid Mustang |
EA02125 |
1×4.6mL |
EA02135 |
5×4.6mL |
|
EzLoad 16/10 Plasmid Mustang |
EA316304 |
1 pcs |
EzLoad 26/10 Plasmid Mustang |
EA316306 |
1 pcs |