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Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Q Bestarose HP (high performance) is a strong anion exchange resin formed by coupling quaternary amine groups on the diamond matrix.
|
Appearance |
White slurry |
|
Matrix |
Highly cross-linked agarose |
|
Functional group |
Quaternary amine group |
|
Particle size+ |
24~44μm (the average particle size is 34μm) |
|
Ionic capacity |
140~200μmol Cl-/mL packed resin |
|
Dynamic binding capacity++ |
~ 75 mg BSA/mL resin |
|
Max. pressure |
0.3MPa |
|
Chemical Stability |
Stable in common aqueous buffers: 0.5M NaOH+++,2M NaOH+++,0.1M HCl, 70% formic acid,6M GuHCl,8M Urea, 30% acetonitrile, 30% isopropyl alcohol, 24% ethanol, 70% ethanol, 50mM PB+2M NaCl pH7.0. Avoid contact with oxidizing agents, cationic detergents. |
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pH stability |
2~14 (CIP),2~12(Working) |
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Operating temperature |
Operating temperature:2~40℃, do not freeze, Can tolerate 121℃ high pressure sterilization (add 50mM PB pH7). |
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Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Recommend flow velocity |
60~150cm/h |
+ Average particle size is the accumulated resin particle size of packing volume distribution
++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load at flow velocity of 1mL /min at 50mM Tris+1M NaCl pH8
+++NaOH only be used for cleaning
++++2% benzyl alcohol is only used for international transport or special requirements from customer
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Product |
Cat. No. |
Pack size |
|
Q Bestarose HP |
AI0071 |
25mL |
|
AI0076 |
100mL |
|
|
AI0072 |
500mL |
|
|
AI0073 |
1L |
|
|
AI0074 |
5L |
|
|
AI0075 |
10L |
|
|
AI204115 |
20L |
|
|
AI204116 |
40L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Q HP |
EI00721 |
1×1mL |
|
EI031 |
5×1mL |
|
|
EI204103 |
1×5mL |
|
|
EI032 |
5×5mL |
|
|
EzScreen Q HP |
EI00725 |
1×4.6mL |
|
EI00735 |
5×4.6mL |
|
|
EzLoad 16/10 Q HP |
EI034 |
1 pcs |
|
EzLoad 26/10 Q HP |
EI035 |
1 pcs |
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