SP Bestarose HP Strong Cation Exchange Resins-IEX Resins丨Bestchrom

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1. Introduction

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.

IEX resin is composed of three parts: (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability. (2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin. (3)An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.

SP Bestarose HP (high performance) is a strong cation exchange resin formed by coupling sulfopropyl groups on fine-grained high-resolution agarose microspheres.

2. Technical characteristics

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional group	Sulfopropyl
Particle size⁺	24~44μm (the average particle size is 34μm)
Ionic capacity	150~200μmol H⁺/mL packed resin
Dynamic binding capacity⁺⁺	~ 70 mg BSA/mL resin
Max. pressure	0.3MPa
Chemical Stability	Stable in common aqueous buffers: 1M NaOH⁺⁺⁺,1M HAC⁺⁺⁺,6M GuHCl, 30%isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents.
pH stability	3~14(CIP), 4~13(working)
Operating temperature	Operating temperature:2~40℃, do not freeze.
Storage⁺⁺⁺⁺	2~30℃, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc
Recommended flow velocity	60-150cm/h

+ Average particle size is the accumulated resin particle size of packing volume distribution.

++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load at 100mM NaHAc pH9 flow velocity of 1mL /min,

+++1M NaOH and 1M HAc only be used for cleaning.

++++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
SP Bestarose HP	AI0080	25mL
	AI0081	100mL
	AI0082	500mL
	AI0083	1L
	AI0084	5L
	AI0085	10L
	AI203115	20L
	AI203116	40L

Prepacked columns	Cat. No.	Pack size
EzFast SP HP	EI00821	1×1mL
	EI036	5×1mL
	EI203103	1×5mL
	EI037	5×5mL
EzScreen SP HP	EI00825	1×4.6mL
EzScreen SP HP	EI00835	5×4.6mL
EzLoad 16/10 SP HP	EI039	1 pcs
EzLoad 26/10 SP HP	EI040	1 pcs