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Blue Bestarose FF is an affinity resin based on agarose and use Cibacron Blue 3GA as ligand. It has the characteristics of physical and chemical stability, low ligand leakage, long service life, and a wide range of applications. This resin can bind proteins through specific interactions, as well as non-specifically bind proteins through charges and hydrophobic interactions. Blue Bestarose FF has been widely used in the separation and purification of various proteins, including dehydrogenase, kinase, transferase, serum albumin, interferon and plasma protein.
|
Appearance |
Blue slurry |
|
Matrix |
Highly cross-linked 6% agarose |
|
Particle size+ |
45~165μm |
|
Functional group |
Cibacron Blue 3GA |
|
Ligand concentration |
~7.3μmol chromophore /mL resin |
|
Dynamic binding capacity |
＞18mg HSA/mL packed resin |
|
Max. pressure |
0.3MPa |
|
pH stability |
4~12(working),3~13 (CIP) |
|
Chemical Stability |
Stable in common aqueous buffers: 8M urea, 6M GuHCl,70% ethanol |
|
Operating temperature |
Operating temperature:2~40℃, do not freeze. |
|
Storage++ |
2~8℃,20% ethanol + 0.1M KH2PO4 (pH 8.0)or 2% benzyl alcohol + 0.1M KH2PO4 (pH 8.0) |
|
Recommended flow velocity |
30-300cm/h |
+Particle size is normally distributed, and particles within this range account for more than 95% of the total
++2% benzyl alcohol is only used for international transport or special requirements from customer
|
Product |
Cat. No. |
Pack size |
|
Blue Bestarose FF |
AA210305 |
25mL |
|
AA210307 |
100mL |
|
|
AA0092 |
500mL |
|
|
AA0093 |
1L |
|
|
AA0094 |
5L |
|
|
AA0095 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Blue FF |
EA018 |
5×1mL |
|
EA019 |
5×5mL |
|
|
EzScreen Blue FF |
EA00925 |
1×4.6mL |
|
EA00935 |
5×4.6mL |
|
|
EzLoad 16/10 Blue FF |
EA210304 |
1 pcs |
|
EzLoad 26/10 Blue FF |
EA210306 |
1 pcs |
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