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Diamond Blue is a kind of affinity resin based on high rigidity agarose with fine particles. The ligand is Cibacron Blue 3GA, which is physically and chemically stable. Since the ligand is not easy to drop off, it can enjoy long service life and a wide range of applications. The resin can bind proteins through specific interactions, as well as non-specific binding through electric charges. Diamond Blue has been widely used in the separation and purification of various proteins, including dehydrogenase, kinase, transferase, serum albumin, interferon and plasma proteins.
Compared to Blue Bestarose FF, Diamond Blue has better pressure flow velocity performance and higher binding capacity, helping to improve process efficiency and reduce cost.
|
Appearance |
Blue slurry |
|
Matrix |
High cross-linked rigidity agarose |
|
Average particle size+ |
~75μm |
|
Functional group |
Cibacron Blue 3GA |
|
Ligand concentration |
~13μmol chromophore /mL resin |
|
Dynamic binding capacity++ |
≥24mg HSA/mL packed resin |
|
Chemical Stability |
40℃ 1 week:10mM HCl,0.1M NaOH, 8M urea, 6M GuHCl 40℃ 12h:1M NaOH, 70% acetic acid |
|
Max. pressure |
0.5MPa |
|
Pressure flow velocity |
≥1200cm/h（0.5MPa BXK 100/500, H=20cm, 20℃） |
|
pH stability |
2~13(working),2~13(CIP) |
|
Temperature tolerance |
Working temperature:2~40℃, Can't freeze. |
|
Storage+++ |
2~8℃,20% ethanol + 0.1M KH2PO4(pH 8.0)or 2% benzyl alcohol + 0.1M KH2PO4(pH 8.0) |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++ Residence time: 4min.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
|
Product |
Cat. No. |
Pack size |
|
Diamond Blue |
AA310305 |
25mL |
|
AA310307 |
100mL |
|
|
AA310311 |
500mL |
|
|
AA310312 |
1L |
|
|
AA310313 |
5L |
|
|
AA310314 |
10L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond Blue |
EA310301 |
1×1mL |
|
EA310303 |
1×5mL |
|
|
EA310351 |
5×1mL |
|
|
EA310353 |
5×5mL |
|
|
EzScreen Diamond Blue |
EA04025 |
1×4.6mL |
|
EA04035 |
5×4.6mL |
|
|
EzLoad 16/10 Diamond Blue |
EA310304 |
1 pcs |
|
EzLoad 26/10 Diamond Blue |
EA310306 |
1 pcs |
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