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Diamond Layer 700 BA is novel mixed-mode purification chromatography resin designed for intermediate purification and polishing purification of viruses and other biomolecules.
The resin has a two-layer structure, nucleic microspheres and a thin shell. The nucleic microspheres are prepared by crosslinking the octylamine ligands to high rigidity agarose, and have a variety of interaction modes. The thin shell is a layer of agarose structure without ligand, which has a molecular sieve function. In the purification process, large molecules such as viruses cannot enter the inside of the microsphere through the shell, so they flow through directly. Most impurities can enter the inside of the microsphere and bind to the resin through ion and hydrophobic interaction. These characteristics make Diamond Layer 400 and Diamond Layer 700 resin an excellent choice for virus purification resin in vaccine production. The size exclusion of outer layer were 400KD and 700KD, respectively. Biomolecules less than the corresponding molecular weight could enter the microsphere and bond with ligand groups, while biomolecules greater than the corresponding molecular weight would be blocked in the microsphere outflow and pass through the chromatography column.
The main characteristics of Diamond Layer 700 BA resin include:
● The double-layer structure of the microspheres enables the resin to effectively adsorb impurities while the target protein flows through to achieve the purification effect.
● Due to the flow through of the target, the impurities are bond in hydrophobic and ionic mode, allowing relatively wide operating conditions.
● Compared with the conventional molecular sieve resin, the high sample loading volume and high flow velocity improve the production efficiency.
|
Matrix |
Highly cross-linked rigidity agarose |
|
Functional group |
Mixed-mode |
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Average particle size+ |
~85μm |
|
Appearance++ |
White to slight yellow slurry |
|
Dynamic binding capacity |
~13mg ovalbumin/mL packed resin |
|
Average molecular weight exclusion |
Mr≈700 000 |
|
Pressure flow velocity |
≥500cm/h(H=20cm,pressure＜2bar) |
|
Chemical Stability |
Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,70% ethanol , 20% ethanol , 30% isopropyl alcohol, 6M GuHCl, 0.5% twain 80, 1M NaOH+30% isopropyl alcohol |
|
pH stability |
3~13(working), 2~14(CIP) |
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Storage++++ |
2~30℃, 20% ethanol or 2% benzyl alcohol |
|
Avoid |
Avoid contact with oxidizing agents, citrate buffer solution. |
+ Average particle size is the accumulated resin particle size of packing volume distribution.
++Due to ligand properties, the color gradually changes from white to light yellow with the increase of storage time, and the color change has no effect on product performance.
+++1M NaOH and 1M HAc only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.
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Product |
Cat. No. |
Pack size |
|
Diamond Layer 700 BA |
AI04901 |
25mL |
|
AI04902 |
100mL |
|
|
AI04903 |
500mL |
|
|
AI04904 |
1L |
|
|
AI04905 |
5L |
|
|
AI04906 |
10L |
|
|
AI04907 |
20L |
|
Prepacked columns |
Cat. No. |
Pack size |
|
EzFast Diamond Layer 700 BA |
EI04921 |
1×1mL |
|
EI04931 |
5×1mL |
|
|
EI04923 |
1×5mL |
|
|
EI04933 |
5×5mL |
|
|
EzScreen Diamond Layer 700 BA |
EI04925 |
1×4.6mL |
|
EI04935 |
5×4.6mL |
|
|
EzLoad 16/10 Diamond Layer 700 BA |
EI04901 |
1 pcs |
|
EzLoad 26/10 Diamond Layer 700 BA |
EI04911 |
1 pcs |
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