GST Bestarose 4FF Affinity Chromatography Resin

GST Bestarose 4FF

Product Characteristics

The chromatographic resin has high pressure resistance, fast flow velocity, and mild operating conditions, which is conducive to the maintenance of protein activity.

GST Bestarose 4B

Benzamidine Bestarose 4FF

Description

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1. Introduction

GST Bestarose 4FF is made by coupling glutathione to a highly cross-linked agarose, which is used to specifically purify glutathione S-Transferase (GST) as well as the purification of GST fusion protein. GST tag is a tag commonly used in modern genetic engineering to express fusion proteins, allowing soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion proteins, High-purity target protein can be obtained in one-step purification when using this resin. The c resin has high pressure resistance, fast flow velocity, and mild operating conditions, which can help with maintenance of protein activity.

2. Technical characteristics

Appearance	White slurry
Matrix	Highly cross-linked agarose, 4%
Particle size⁺	45-165μm
Functional group	Glutathione with 10 atomic arms
Ligand concentration	120~320μmol Glutathione /mL resin
Dynamic binding capacity	~10mg GST/mL packed resin
Max. pressure	0.3MPa
Chemical Stability	Stable in common aqueous buffers: 1M HAc⁺⁺, 70%ethanol, 6M GuHCl (1 hour at room temperature)
pH stability	3~12
Storage⁺⁺⁺	2~30℃, 20%ethanol or 2% benzyl alcohol
Pressure flow velocity	~450cm/h, BXK16/10,H=5cm,25℃
Recommended flow velocity	Sample loading flow velocity:＜100cm/h Equilibration, cleaning, elution flow velocity:100~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.

++1M HAc only be used for cleaning.

+++2% benzyl alcohol is only used for international transport or special requirements from customer.

3. Order information

Product	Cat. No.	Pack size
GST Bestarose 4FF	AA0071	25mL
	AA0072	100mL
	AA0073	500mL
	AA0074	1L
	AA0075	5L
	AA109314	10L

Prepacked columns	Cat. No.	Pack size
EzFast GST 4FF	EA109301	1×1mL
	EA109303	1×5mL
	EA012	5×1mL
	EA013	5×5mL
EzScreen GST 4FF	EA00725	1×4.6mL
EzScreen GST 4FF	EA00735	5×4.6mL
EzLoad 16/10 GST 4FF	EA109304	1 pcs
EzLoad 26/10 GST 4FF	EA109306	1 pcs